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 香川共同リポジトリは、香川県内で生産された学術研究成果を電子的に収集、整 理、保存し、インターネットを通じて無償で提供することを目的として公開して います。

*平成23年度国立情報学研究所最先端学術情報基盤(CSI)構築推進委託事業です。

kuir is 6340
total is 6905
小学校教員養成「初等音楽」への鑑賞活動の導入とその効果 青山 夕夏 Yuka Aoyama 岡田 涼 Ryo OKADA


「外国語活動」直前の英会話授業からの一考察 中住 幸治 Yukiharu Nakazumi


社会科教育におけるアクティブラーニング型授業開発に関する研究―「真正の学び」との関係性に着目して― 鈴木 正行 Masayuki SUZUKI 髙倉 良一 Ryoichi TAKAKURA} 守田 逸人 Hayato MORITA 池田 良 Ryo IKEDA 小野 智史 Tomofumi ONO 大和田 俊 Syun Ohwada 山城 貴彦 Takahiko YAMASHIRO


児童・生徒からの援助要請に対する教師の必要性認知に関する研究 岡田 涼 Ryo OKADA 池田 七海 Nanami IKEDA


Acetaldehyde administration induces salsolinol formation in vivo in the dorsal striatum of Aldh2-knockout and C57BL/6N mice 伊藤 明日香 Ito Asuka
Abstract Acetaldehyde (AcH) and salsolinol play important roles in the central effects of ethanol. This study aimed to investigate the effect of administration of AcH on dopamine (DA), DA-derived salsolinol and serotonin (5-HT) levels in the dorsal striatum of Aldh2-knockout (Aldh2-KO) and C57BL/6 N (WT) mice. Animals were treated with AcH (50, 100 and 200 mg/kg) intraperitoneally and dialysate levels of DA, 5-HT and salsolinol were determined using in vivo microdialysis coupled with HPLC-ECD. Salsolinol was first detected at 20 min after AcH administration, and reached its peak concentration (WT mice: 0.29 ± 0.22 pg/μl; Aldh2-KO mice: 0.63 ± 0.17 pg/μl) at 25 min in the 200 mg/kg AcH group, before decreasing rapidly and reaching zero at approximately 55-80 min. Treatment with 100 and 200 mg/kg AcH increased levels of salsolinol in both WT and Aldh2-KO mice, with 200 mg/kg AcH inducing a higher level of salsolinol in Aldh2-KO mice than in WT mice. Treatment with 50 mg/kg AcH produced a small increase in salsolinol levels in Aldh2-KO mice, whereas no elevation of salsolinol was detected in WT mice. The increase in salsolinol formation was found to occur a dose-dependent manner in both genotypes. Administration of AcH and the subsequent changes in salsolinol concentrations did not change DA or 5-HT levels in either genotype. Our study suggests that AcH dose-dependently increases the formation of salsolinol in the dorsal striatum of mice, which provides further support for the role of AcH in salsolinol formation in the animal brain. KEYWORDS: Acetaldehyde; Aldh2-KO mice; Microdialysis; Salsolinol; Striatum (医博甲716)

The role of intracellular anionic phospholipids in the production of N-acyl-phosphatidylethanolamines by cytosolic phospholipase A2ε Smriti Sultana Binte Mustafiz
Abstract N-Acyl-phosphatidylethanolamines (NAPEs) represent a class of glycerophospholipids and serve as the precursors of bioactive N-acylethanolamines, including arachidonoylethanolamide (anandamide), palmitoylethanolamide and oleoylethanolamide. NAPEs are produced in mammals by N-acyltransferases, the enzymes which transfer an acyl chain of glycerophospholipids to the amino group of phosphatidylethanolamine. Recently, the ɛ isoform of cytosolic phospholipase A2 (cPLA2ɛ, also called PLA2G4E) was identified as Ca2+-dependent N-acyltransferase. We showed that the activity is remarkably stimulated by phosphatidylserine (PS) in vitro. In the present study, we investigated whether or not endogenous PS regulates the function of cPLA2ɛ in living cells. When PS synthesis was suppressed by the knockdown of PS synthases in cPLA2ɛ-expressing cells, the cPLA2ɛ level and its N-acyltransferase activity were significantly reduced. Mutagenesis studies revealed that all of C2, lipase and polybasic domains of cPLA2ɛ were required for its proper localization as well as the enzyme activity. Liposome-based assays showed that several anionic glycerophospholipids, including PS, phosphatidic acid and phosphatidylinositol 4,5-bisphosphate, enhance the Ca2+-dependent binding of purified cPLA2ɛ to liposome membrane and stimulate its N-acyltransferase activity. Altogether, these results suggested that endogenous PS and other anionic phospholipids affect the localization and enzyme activity of cPLA2ɛ. KEYWORDS: N-acyl-phosphatidylethanolamine; N-acylethanolamine; N-acyltransferase; lipid mediator; phosphatidylserine (医博甲708)

疑似乱数とカオス 久松 博之 Hiroyuki Hisamatsu


The usefulness of pleural fluid presepsin, C-reactive protein, and procalcitonin in distinguishing different causes of pleural effusions 渡邊 直樹 Watanabe Naoki
Abstract BACKGROUND: We aimed to determine the presepsin concentration in pleural fluid from patients with pleural effusions of different aetiologies and to compare its diagnostic value with that of pleural fluid C-reactive protein (CRP) and procalcitonin (PCT). METHODS: We enrolled 132 patients with pleural effusion who underwent diagnostic evaluation, and we classified them into six categories: empyema, parapneumonic effusion, tuberculous effusion, malignant effusion, paramalignant effusion, and transudate effusion. Additionally, all pleural effusions were categorised as infectious or non-infectious effusions. RESULTS: Receiver operating characteristic analysis was used to evaluate diagnostic performance. When diagnosing empyema, the marker with the highest sensitivity was pleural fluid presepsin (cut-off: 754 pg/mL; sensitivity: 90.9%, specificity: 74.4%) and that with the highest specificity was pleural fluid CRP (cut-off: 4.91 mg/dL; sensitivity: 63.6%, specificity: 89.3%). Pleural fluid PCT tended to be lower in patients with empyema than in those with parapneumonic effusion, but this was not useful for the diagnosis of empyema. When diagnosing infectious pleural effusion, a combination of pleural fluid CRP (cut-off: 2.59 mg/dL) and presepsin (cut-off: 680 pg/mL) produced the highest diagnostic accuracy (83.3%). CONCLUSIONS: Pleural fluid presepsin was found at high levels in patients with empyema and parapneumonic effusion. This pattern closely resembles the previously reported pattern of pleural fluid CRP. Some combinations of pleural fluid inflammatory markers may be more clinically useful than these markers in isolation. KEYWORDS: C-reactive protein; Empyema; Pleural effusion; Presepsin; Procalcitonin (医博甲721)

Chest Compression Depth and Rate - Effect on Instructor Visual Assessment of Chest Compression Quality 永渕 克弥 Nagafuchi Katsuya
Abstract BACKGROUND: The aim of the present study was to determine whether instructors could accurately assess chest compression quality visually, considering the association between chest compression depth and rate. Methods and Results: In this prospective, observational study, the quality of chest compressions performed by a simulated actor in a video was visually assessed by certified instructors. The film consisted of 14 case scenarios, each including a combination of depth (2 patterns: adequate, 5-6 cm; and inadequate, <5 cm) and rate (7 categories: compressions 90-150 times/min in increments of 10 times/min). The participants evaluated whether the compression depth was adequate, deep, or inadequate; and whether the compression rate was appropriate, fast, or slow. Of 198 instructors, 56% of participants misidentified adequate depth as deep at a chest compression rate of 120/min (the tendency toward this response increased as chest compression rate increased), and 64.1% of participants incorrectly determined 130/min to be appropriate. On generalized linear mixed-effects model analysis, perceived chest compression depth and rate were significant factors for a correct response (P<0.01, both). A significant interaction between chest compression depth and rate was observed (P<0.01). CONCLUSIONS: In the visual assessment of chest compression quality, recognition of chest compression depth was closely associated with compression rate. Misidentification of adequate chest compression depth as deep increased as the compression rate increased. KEYWORDS: Basic science; Cardiopulmonary resuscitation; Chest compression resuscitation (医博甲720)

Histomorphometry of ectopic mineralization using undecalcified frozen bone sections 藤原 龍史 Fujihara Ryuji
Abstract To investigate the correlation between mineral formation and enhanced expressions of some proteins using undecalcified frozen bone sections. Histological studies have revealed that some proteins, such as BMP2, BMPR1A, and Connexin 43, are expressed in and around sites of ectopic ossification. However, the relationship between the expressed proteins considered to be associated with the ossification and mineral formation in vivo is not clear. Ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1)-mutant spinal hyperostotic TWY mice and ICR mice as controls were euthanized after calcein labeling, and undecalcified frozen sections were obtained from the middle thoracic spine. Intervertebral disc areas were examined histologically and by measuring calcein-labeled areas and areas showing immunoreactivity for BMP2, BMPR1A, and Connexin 43. Calcein-labeled areas, indicating mineralization in the ectopic mineralization sites, were significantly larger in the mutant mice than in controls. The expression of Connexin 43 was elevated in the annulus fibrosus. Increases in the calcein-labeled areas was not correlated with increases in the areas showing immunoreactivity for Connexin 43 in the annulus fibrosus. There was no statistical correlation between enhanced immunohistochemical expression and elevated calcein-labeled areas. By applying the morphometrical analysis method using undecalcified frozen sections to ENPP1-mutant mice, quantitative evaluation of the mineralization and proteins expressed in the surrounding area in the same animal became possible. KEYWORDS: BMP2; BMPR1A; Connexin 43; ENPP1; histomorphometry; mineralization; ossification of the posterior longitudinal ligament; undecalcified frozen section (医博甲719)

プロスポーツチーム組織におけるフロントスタッフの役割と仕事:「ホームゲーム」の産出に着目して 宇野 博武


研究授業「教育学原論」の報告 相馬 宗胤


研究授業「文書実務」の実施報告 佐藤 麻衣


研究授業「医療秘書概論」の実施報告 秋鹿 悦子


小学校外国語指導法について~「気付き」を促す指導~ 竹田 忠弘


生産性向上についての一考察―日本企業を対象として― 岡本 丈彦


研究授業「コミュニケーション演習Ⅱ」の実施報告 森 享子 蓮井 孝夫


Google Classroomを用いた授業管理の利点と問題点 山口 直木


プロスポーツ経営研究の動向と課題 宇野 博武


情報モラル教育の現状を踏まえた、情報機器を活用した情報モラル教育環境の構築について 佃 昌道