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 香川共同リポジトリは、香川県内で生産された学術研究成果を電子的に収集、整 理、保存し、インターネットを通じて無償で提供することを目的として公開して います。

*平成23年度国立情報学研究所最先端学術情報基盤(CSI)構築推進委託事業です。

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Long-Term Management of Recurrent Cholecystitis after Initial Conservative Treatment: Endoscopic Transpapillary Gallbladder Stenting 鎌田 英紀 Kamada Hideki
Abstract Background: Endoscopic transpapillary gallbladder stenting (ETGBS) is an effective procedure for treating high-risk patients with acute cholecystitis and severe comorbidities. However, the efficacy of ETGBS for recurrent cholecystitis (RC) remains unclear. This study aimed to explore its efficacy in patients with RC for whom cholecystectomy is contraindicated because of its high surgical risk. Methods: Data on 19 high-risk patients who had undergone ETGBS for RC after initial conservative therapy in our institution between June 2006 and May 2012 were retrospectively examined. The primary outcome was the clinical success rate, which was defined as no recurrences of acute cholecystitis after ETGBS until death or the end of the follow-up period. Secondary outcomes were technical success rate and adverse events (AEs). Results: The clinical success rate of ETGBS was 100%, the technical success rate 94.7%, and AE rate 5%: one patient developed procedure-related mild acute pancreatitis. The clinical courses of all patients were as follows: four died of nonbiliary disease, and the remaining 15 were subsequently treated conservatively. The median duration of follow-up was 14.95 months (range 3-42 months). Conclusions: ETGBS is an effective alternative for managing RC in high-risk patients with severe comorbidities.

Normobaric oxygen therapy increases cartilage survival ratio in auricular composite grafting in rat models 濵本 有祐 Hamamoto Yusuke
Abstract: Purpose: This study aims to clarify whether normobaric oxygen therapy improves the survival of auricular composite grafts in rats. Methods: For 10 male SD rats, 1.5 cm2 composite grafts were harvested from bilateral ear regions including whole auricles. The harvested grafts were transferred caudally and sutured there. The 10 rats were randomly divided into two groups and kept for 21 days in two different circumstances. The first group (Control group: five rats carrying 10 grafts) was kept in room air (20% oxygen) throughout the 21 days, and the second group―named NBO (normobaric oxygen) group (five rats carrying 10 grafts)―was kept in normobaric 60% oxygen for 3 days and then in room air for 18 days. All the 10 rats were sacrificed on the 21st day. Surviving areas of the grafts and the height of the surviving auricular cartilage were examined for statistical comparison of the two groups. Furthermore, the conditions of chondrogenesis occurring around the perichondrium were compared between the two groups. Results: Surviving areas did not present statistically significant differences between the two groups. The height of surviving cartilage was significantly greater for the NBO group (2610 ± 170 SD µm) than that for the Control group (1720 ± 190 SD µm). Chondrogenesis occurred at positions more distant from the recipient bed in the NBO group than that in the Control group. Conclusion: Normobaric oxygen therapy increases the thickness of surviving cartilage in auricular composite grafting in rats, thus suggesting that NBO therapy may also be effective in composite grafting for humans. © 2018 The Authors Keywords: Auricle; Blood; Cartilage; Composite graft; Ear;

Characterization of a recombinant Bacteroides fragilis sialidase expressed in Escherichia coli 山本 高成 Yamamoto Takaaki
Abstract The human gut commensal Bacteroides fragilis produces sialidases that remove a terminal sialic acid from host-derived polysaccharides. Sialidase is considered to be involved in B. fragilis infection pathology. A native B. fragilis sialidase has been purified and characterized, and was shown to be post-translationally modified by glycosylation. However, the biochemical properties of recombinant B. fragilis sialidase expressed in a heterologous host remain uncharacterized. In this study, we examined the enzymatic properties of the 60-kDa sialidase NanH1 of B. fragilis YCH46, which was prepared as a recombinant protein (rNanH1) in Escherichia coli. In E. coli rNanH1 was expressed as inclusion bodies, which were separated from soluble proteins to allow solubilization of insoluble rNanH1 in a buffer containing 8 M urea and renaturation in refolding buffer containing 100 mM CaCl2 and 50 mM L-arginine. The specific activity of renatured rNanH1 measured using 4-methylumberiferyl-α-D-N-acetyl neuraminic acid as a substrate was 6.16 μmol/min/mg. The optimal pH of rNanH1 ranged from 5.0 to 5.5. The specific activity of rNanH1 was enhanced in the presence of calcium ions. rNanH1 preferentially hydrolyzed the sialyl α2,8 linkage and cleaved sialic acids from mucin and serum proteins (e.g., fetuin and transferrin) but not from α1-acid glycoprotein, which is similar to the previously observed biochemical properties for a native sialidase purified from B. fragilis SBT3182. The results and methods described in this study will be useful for preparing and characterizing recombinant proteins for other B. fragilis sialidase isoenzymes. KEYWORDS: Bacteroides fragilis; Mucin; Sialic acid; Sialidase; Sialyl α2,8 linkage

IGF1 suppresses cholesterol accumulation in the liver of growth hormone deficient mice via the activation of ABCA1 福長 健作 Fukunaga Kensaku
Abstract Recently, several clinical studies have suggested that adult growth hormone deficiency that also has low concentration of IGF1 is associated with an increased prevalence of fatty liver (FL). ABCA1 is a pivotal regulator of lipid efflux from cells to apolipoproteins and play an important role on formation of FL. In this study, we determined the effects of IGF1 on ABCA1 expression in GH deficient mice to clarify its effects on FL. Western blotting, real-time PCR and a luciferase assay were employed to examine the effect of IGF1. The binding of FoxO1 to the ABCA1 promoter was assessed by ChIP assay. Cholesterol accumulation was analyzed by Oil-Red-O stain and cholesterol content measurement. We confirmed that IGF1 up-regulated the ABCA1 expression. The activity of a reporter construct containing the ABCA1 promoter was induced by IGF1, and this effect was blocked by LY294002, a specific inhibitor of PI3K. Constitutively active Akt stimulated the ABCA1 promoter activity, and a dominant-negative mutant of Akt or mutagenesis of the FoxO1 response element abolished the effect of IGF1. A ChIP assay indicated that FoxO1 mediated IGF1 transcriptional activity by directly binding to the ABCA1 promoter region. In vivo experiments, we used an inhibitor for the GH receptor (Pegvisomant) to reduce the IGF1 level. A HFD induced FL in mice (C57BL/6J) given Pegvisomant-treat. IGF1-treatment stimulated ABCA1 expression to improve cholesterol accumulation in these mice. These results show that the PI3K/Akt/FoxO1 pathway contributes to the regulation of ABCA1 expression in response to IGF1-stimulation that suppressed FL in GH-deficient mice. KEYWORDS: ABCA1; Adult growth hormone deficiency; Fatty Liver; IGF1

SNX-2112 Induces Apoptosis and Autophagy of Nara-H Cells 福岡 奈津子 Fukuoka Natsuko
Abstract BACKGROUND/AIM: Selective heat shock protein 90 (Hsp90) inhibitor SNX-2112 exhibits antitumor activity in multiple cancer cell types. Here, the antitumor activity of SNX-2112 in Nara-H cells was analyzed. MATERIALS AND METHODS: Antitumor activity of SNX-2112 was assessed using a cell proliferation assay. We also examined the signalling pathways involved in SNX-2112-mediated autophagy and apoptosis of Nara-H cells by western blot and morphological analyses. RESULTS: Cell proliferation assays demonstrated that SNX-2112 inhibited Nara-H cell growth. Western blotting revealed that SNX-2112 induced apoptosis and autophagy, inhibited mammalian target of rapamycin (mTOR) phosphorylation, and suppressed the mitogen-activated protein kinase (MAPK) signalling pathway. Morphological analysis confirmed that SNX-2112 induced autophagy and apoptosis. CONCLUSION: SNX-2112 induced autophagy and apoptosis of Nara-H cells by inhibiting mTOR and MAPK pathways. Our results support developing SNX-2112 to treat human soft tissue sarcomas. KEYWORDS: SNX-2112; apoptosis; autophagy; undifferentiated pleomorphic sarcoma

医学部キャンパス報告 高橋 岳水 Takemi Takahashi 川田 延枝 Nobue Kawada 多田 志乃舞 Shinobu Tada


ユーザーサービス部門活動報告 米谷 雄介 Yusuke Kometani 最所 圭三 Keizo Saisho 後藤田 中 Naka Gotoda 近藤 まゆみ Mayumi Kondo 小野 滋己 Shigemi Ono 山下 俊昭 Toshiaki Yama 豊嶋 尚子 Naoko Toyoshima 末光 京子 Kyoko Suemitsu 青木 有香 Yuka Aoki 香西 理恵 Rie Kozai 七條 強 Tsuyoshi Shichijo


情報セキュリティ部門報告 後藤田 中 Naka Gotoda 米谷 雄介 Yusuke Kometani 小野 滋己 Shigemi Ono 青木 有香 Yuka Aoki 福家 隆 Takashi Fuke 高橋 岳水 Takemi Takahashi 川口 政秀 Masahide Kawaguchi 土居 敬典 Takanori Doi 山下 俊昭 Toshiaki Yamashita 末廣 紀史 Norihumi Suehiro


香川大学ネットワークシステムの今昔 今井 慈郎 Yoshiro Imai 山下 俊昭 Toshiaki Yamashita 川口 政秀 Masahide Kawaguchi 土居 敬典 Takanori Doi 高橋 岳水 Takemi Takahashi


教育システム部門活動報告~2017年度頑張ったこと、将来のこと~ 末廣 紀史 Norihumi Suehiro 近藤 まゆみ Mayumi Kondo 川口 政秀 Masahide Kawaguchi 末光 京子 Kyoko Suemitsu 香西 理恵 Rie Kouzai 髙橋 岳水 Takemi Takahashi 土居 敬典 Takanori Doi 八重樫 理人 Rihito Yaegashi


Google Classroomを用いた授業管理の利点と問題点 山口 直木


プロスポーツ経営研究の動向と課題 宇野 博武


情報モラル教育の現状を踏まえた、情報機器を活用した情報モラル教育環境の構築について 佃 昌道


「チームとしての学校」における「協働」についての予備的考察 佐竹 勝利


実践教育に重きを置いた情報科教育法 -教育実習の視点からの展開- 佃 昌道


幼稚園等における教育相談の実際から―学生に伝えるべき学びの考察― 佐々木 利子 有馬 則子


魅力ある環境づくりと保育者の役割―幼児教育の独自性から科目「保育内容―環境」を考える 藤澤 典子 佐々木 利子


子どもの言葉を豊かに育てるための保育者の役割ー子どもの貧困対策につながる就学前教育の効果を参考して― 山本 幾代


教育実習等の指導内容に関する考察 田中 弓子 佐々木 利子


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