ゼアラレノンに対するモノクローナル抗体の作製

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/1278
Title
ゼアラレノンに対するモノクローナル抗体の作製
Title Alternative
Production of monoclonal antibodies against zearalenone
File
Description

In order to establish immunological analytical methods for zearalenone(ZEN), worldwide occurring in cereal grains and feeds contaminated Fusarium graminearum, specific monoclonal antibodies (mAbs) against ZEN were newly prepared. Spleen cells from the mice immunized with ZEN-bovine serum albumin were fused with SP2/O myeloma. After HAT selection and cloning, two anti-ZEN antibody producing hybridomas (ZEN.1 and 2) were obtained. In the indirect competitive ELISA with ZEN. 2, the limit of detection was 0.25 ng/mL. The ZEN.2 mAb cross-reacted with α-zearalenol (60%), β-zearalenol (5.7%), α-zearalanol (7.1%), and β-zearalanol (0.9%). Our ELISA was sensitive and specific previously reported ELISAs.

Author
著者 川村 理
著者(ヨミ) カワムラ オサム
著者(別表記) Kawamura Osamu
著者 江本 知朗
著者(ヨミ) エモト アキオ
著者(別表記) Emoto Akio
Publication Title
香川大学農学部学術報告
Volume
58
Start Page
7
End Page
11
Publisher
香川大学農学部
Published Date
20060215
ISSN
0368-5128
NCID
AN00038339
Resource Type
Departmental Bulletin Paper
Language
jpn
Text Version
publisher
Set
香川大学
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