イムノアフィニティーカラム-HPLC法によるウシ尿中のゼアラレノンの測定

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/1294
Title
イムノアフィニティーカラム-HPLC法によるウシ尿中のゼアラレノンの測定
Title Alternative
Determination of Zearalenone in Bovine Urine by an Immunoaffinity Column-HPLC Method
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Description

In order to establish an immunoaffinity column-HPLC method for zearalenone (ZEN) in bovine urine, purified anti-ZEN monoclonal antibody (ZEN.2) was bound to Affi-Gel 10 gel, and the antibody-binding gel was packed into a mini column. When this column was applied with 10 ml of ZEN,α-zearalenol, β-zearalenol, α-zearalanol, and β-zearalanol(100 ng/ml in PBS), these recoveries were 100%, 99.0%, 56.3%,69.9%,and 32.3%, respectively. The recoveries±SD (%) from the bovine urine spiked with 0.1, 0.5, and 2.5 ng/ml of ZEN were 99.6±9.7, 106.2±1.4, and 107.9±1.7 (n=3), respectively, in our immunoaffinity column-HPLC method.

Author
著者 川村 理
著者(ヨミ) カワムラ オサム
著者(別表記) Kawamura Osamu
著者 江本 知朗
著者(ヨミ) エモト アキオ
著者(別表記) Emoto Akio
Publication Title
香川大学農学部学術報告
Volume
59
Start Page
93
End Page
97
Publisher
香川大学農学部
Published Date
20070228
ISSN
0368-5128
NCID
AN00038339
Resource Type
Departmental Bulletin Paper
Language
jpn
Text Version
publisher
Set
香川大学
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