T-2トキシンに対するモノクローナル抗体の作製

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/1295
Title
T-2トキシンに対するモノクローナル抗体の作製
Title Alternative
Production Monoclonal Antibodies Against T-2 Toxin
File
Description

In order to establish immunological analytical methods for T-2 toxin (T-2), occurring in cereal grains and feeds contaminated Fusarium sporotrichioides, specific monoclonal antibodies (mAbs) against T-2 were prepared. Spleen cells from the mice immunized with T-2 hemiglutarate -bovine serum albumin were fused with SP2/O myeloma. After HAT selection and cloning, two anti-T-2 antibody producing hybridomas (T-2.01 and T-2.02) were obtained. In the indirect competitive ELISA with T-2.02, the limit of detection was 20 ng/mL. The T-2.02 mAb cross-reacted with HT-2 (36%), not with neosolaniol and diacetoxyscirpenol. However, the limit of detection in our ELISA was 200-fold higher than previously reported ELISAs.

Author
著者 川村 理
著者(ヨミ) カワムラ オサム
著者(別表記) Kawamura Osamu
著者 上田 恭子
著者(ヨミ) ウエダ キョウコ
著者(別表記) Ueda Kyouko
著者 秋和 誠
著者(ヨミ) アキワ マコト
著者(別表記) Akiwa Makoto
Publication Title
香川大学農学部学術報告
Volume
59
Start Page
99
End Page
104
Publisher
香川大学農学部
Published Date
20070228
ISSN
0368-5128
NCID
AN00038339
Resource Type
Departmental Bulletin Paper
Language
jpn
Text Version
publisher
Set
香川大学
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