THE DETECTION OF OCHRATOXIN A IN COMMERCIAL 'FUSHI' PRODUCTS, WHICH ARE DRIED BONITO, MACKEREL AND HOUSE MACKEREL, BY THE IMPROVEMENT DIRTHYLAMINOPROPYL SILICA GEL SOLID PHASE EXTRACTION METHOD

( Max 2000 Items )
URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/1306
Title
THE DETECTION OF OCHRATOXIN A IN COMMERCIAL 'FUSHI' PRODUCTS, WHICH ARE DRIED BONITO, MACKEREL AND HOUSE MACKEREL, BY THE IMPROVEMENT DIRTHYLAMINOPROPYL SILICA GEL SOLID PHASE EXTRACTION METHOD
Title Alternative
改良ジエチルアミノプロピルシリル化シリカゲル固相抽出法による市販節製品(鰹節,さば節,アジ節)中のオクラトキシンAの検出
File
Description

The improvement diethylaminopropyl silica gel solid phase extraction method, which was simple, low cost and not need toxic organic solvent, good for detection of ochratoxin A (OTA) in foods. This method was partially modified and applied to determine OTA in `fushi' product. The mean recovery of OTA was 85% in the range 1 - 10 ng/g. The limit of detection was 0.5 ng/g. `Fushi' products on the market in 2003 ? 2005 were collected and analyzed by this method. In 49 samples of powdered `kezuri-bushi', 7 samples (17%) were positive (mean 4.1 ng/g). In 12 samples of `dashi-pack', 3 samples (25%) were positive (mean 8.7 ng/g). But 53 samples of `kezuri-bushi' samples were negative (<0.5 ng/g). The results were suggested that `fushi' products were not contaminated in inoculated mold steps, probably in neglecting steps after smoking.

改良ジエチルアミノプロピルシリル化シリカゲル固相抽出法によるオクラトキシンA(OTA)の分析法は,簡便かつ低コストで毒性の強い有機溶媒を使わない優れた方法である.しかし,この方法をそのまま節製品に適用させた場合,回収率が約65%と低かった.そこで,固相抽出での洗浄を80%メタノール:酢酸(99+1)から(99.5+0.5)に変更したところ,85%以上の高回収率であった.本法で,2003-05年の市販節製品114検体を分析した結果,粉末削り節49検体中7検体(17%)平均4.1 ng/gとだしパック12検体中3検体(25%)平均8.7 ng/gのOTAを検出した.しかし,削り節53検体からはOTAは検出されなかった.以上の結果から,節製品の製造工程からOTA汚染は,カビ付け工程以前で起こっている可能性が示唆された.

Author
著者 Kawamura Osamu
著者(ヨミ) カワムラ オサム
著者(別表記) 川村 理
著者 Yamamoto Junko
著者(ヨミ) ヤマモト ジュンコ
著者(別表記) 山本 純子
著者 Syutou Asuka
著者(ヨミ) シュトウ アスカ
著者(別表記) 首藤 明日香
Publication Title
香川大学農学部学術報告
Volume
60
Start Page
83
End Page
86
Publisher
香川大学農学部
Published Date
20080222
ISSN
0368-5128
NCID
AN00038339
Resource Type
Departmental Bulletin Paper
Language
eng
Text Version
publisher
Set
香川大学
Copyright (C) 2009 Kagawa University All rights reserved.