Production of monoclonal antibody against Epstein-Barr virus early antigen and its application to detection of antitumor promoting activity

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/1321
Title
Production of monoclonal antibody against Epstein-Barr virus early antigen and its application to detection of antitumor promoting activity
Title Alternative
Epstein-Barrウイルス早期抗原に対するモノクローナル抗体の作製とその抗発癌プロモーター活性検出への応用
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Description

Hybridoma cell line, designated 2D3-E7, Producing a monoclonal antibody against 48 kDa-early antigen of Epstein-Barr virus was established by the fusion of mouse myeloma cells with spleen cells from a mouse immunized with P3HR-1 cells treated with sodium n-butyrate (1 mM) and a tumor promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA, 40 ng/ml). The antibody was purified from the culture supernatant by precipitation with ammonium sulfate and column chromatographies on Sephadex G-200, Protein G-Sepharose, and Mono Q. Immunoblotting analysis showed that the purified antibody conjugated with horseradish peroxidase reacted with TPA (80 ng/ml) -induced polypeptide of 48 kDa in Raji cells and its induction was inhibited by curcumin (10 μg/ml), an antitumor promoter from turmeric. These results indicated that the immunoblotting analysis can be used in a confirmation test for detection of antitumor promoting activity using the monoclonal antibody conjugated with the peroxidase. Furthermore, adhesion of Raji cells caused by treatment of TPA was inhibited by the curcumin in a dose-dependent manner, suggesting that the inhibition of adhesion can be also used in a preliminary test for detection of antitumor promoting activity.

Author
著者 岡崎 勝一郎
著者(ヨミ) オカザキ カツイチロウ
著者(別表記) Okazaki Katsuichiro
著者 森本 哲弘
著者(ヨミ) モリモト テツヒロ
著者(別表記) Morimoto Tetsuhiro
著者 石川 奈穂子
著者(ヨミ) イシカワ ナホコ
著者(別表記) Ishikawa Nahoko
Publication Title
香川大学農学部学術報告
Volume
56
Start Page
23
End Page
27
Publisher
香川大学農学部
Published Date
20040315
ISSN
0368-5128
NCID
AN00038339
Resource Type
Departmental Bulletin Paper
Language
eng
Text Version
publisher
Set
香川大学
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