Production of monoclonal antibody against Epstein-Barr virus early antigen and its application to detection of antitumor promoting activity

( 最大 2000 件 )
URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/1321
タイトル
Production of monoclonal antibody against Epstein-Barr virus early antigen and its application to detection of antitumor promoting activity
タイトル(別表記)
Epstein-Barrウイルス早期抗原に対するモノクローナル抗体の作製とその抗発癌プロモーター活性検出への応用
ファイル
内容記述

Hybridoma cell line, designated 2D3-E7, Producing a monoclonal antibody against 48 kDa-early antigen of Epstein-Barr virus was established by the fusion of mouse myeloma cells with spleen cells from a mouse immunized with P3HR-1 cells treated with sodium n-butyrate (1 mM) and a tumor promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA, 40 ng/ml). The antibody was purified from the culture supernatant by precipitation with ammonium sulfate and column chromatographies on Sephadex G-200, Protein G-Sepharose, and Mono Q. Immunoblotting analysis showed that the purified antibody conjugated with horseradish peroxidase reacted with TPA (80 ng/ml) -induced polypeptide of 48 kDa in Raji cells and its induction was inhibited by curcumin (10 μg/ml), an antitumor promoter from turmeric. These results indicated that the immunoblotting analysis can be used in a confirmation test for detection of antitumor promoting activity using the monoclonal antibody conjugated with the peroxidase. Furthermore, adhesion of Raji cells caused by treatment of TPA was inhibited by the curcumin in a dose-dependent manner, suggesting that the inhibition of adhesion can be also used in a preliminary test for detection of antitumor promoting activity.

著者
著者 岡崎 勝一郎
著者(ヨミ) オカザキ カツイチロウ
著者(別表記) Okazaki Katsuichiro
著者 森本 哲弘
著者(ヨミ) モリモト テツヒロ
著者(別表記) Morimoto Tetsuhiro
著者 石川 奈穂子
著者(ヨミ) イシカワ ナホコ
著者(別表記) Ishikawa Nahoko
掲載誌
香川大学農学部学術報告
56
開始ページ
23
終了ページ
27
出版者
香川大学農学部
出版年月日
20040315
ISSN
0368-5128
NCID
AN00038339
資料タイプ
紀要論文
言語
英語
出版社版
区分
香川大学
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