イムノアフィニティーカラム-HPLC法によるカカオ加工品中のアフラトキシンの測定

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/1464
Title
イムノアフィニティーカラム-HPLC法によるカカオ加工品中のアフラトキシンの測定
Title Alternative
DETERMINATION OF AFLATOXINS IN CACAO PRODUCTS BY IMMUNOAFFINITY COLUMN-HPLC METHODS
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Description

In order to establish an immunoaffinity column-HPLC method for aflatoxins (AFs) in cocoa powder and cocoa, anti-AFB1 monoclonal antibody (AF.2) was bound to Affi-Gel 10 gel (16 mg/ml), and the antibody-binding gel was packed into a mini column. Cocoa powder (10 g) spiked AFs were extracted with 50 ml of acetonitrile : water (60+40). The extact were 10-fold diluted with PBS and cleaned-up by the IAC. These recoveries of 1 and 3 ng of AFs/g were 72.3~102.0% and RSD were 3.3~6.6%. But when 10 ng AFs/g were added, these recoveries were 62.9~70.7% and RSD were 6.7~22.3%. Cocoa (10 g) spiked 6 ng of AFs/g were extracted with 50 ml of acetonitrile : water : methanol (60+40+20). The extact were 20-fold diluted with PBS and cleaned-up by the IAC. These recoveries of AFB1 and B2 were 73.4 and 82.3% and RSD were 5.5 and 3.4%, respectively. But these recoveries of AFG1 and G2 were less than 45% and RSD were about 9%. Three cocoa powder and 13 cocoa samples on the market were analyzed in this method. All cocoa powder samples were contaminated 0.40~0.94 ng of AFs/g. One cocoa samples were 0.1 ng of AFG1/g.

Author
著者 川村 理
著者(ヨミ) カワムラ オサム
著者(別表記) Kawamura Osamu
著者 濱田 あゆみ
著者(ヨミ) ハマダ アユミ
著者(別表記) Hamada Ayumi
Publication Title
香川大学農学部学術報告
Volume
61
Start Page
85
End Page
88
Publisher
香川大学農学部
Published Date
20090225
ISSN
0368-5128
NCID
AN00038339
Resource Type
Departmental Bulletin Paper
Language
jpn
Text Version
publisher
Set
香川大学
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