Characterization of a sabinene synthase gene from rough lemon (Citrus jambhiri)

( Max 2000 Items )
URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/2448
Title
Characterization of a sabinene synthase gene from rough lemon (Citrus jambhiri)
File
Description

We previously isolated two putative monoterpene synthase genes, RlemTPS1 and RlemTPS2, from rough lemon (Citrus jambhiri) and showed that gene expression of RlemTPS2 was induced by microbial attack. The protein product of RlemTPS2 was obtained using a prokaryotic expression system, and GC and GC-MS of monoterpene synthesis by RlemTPS2 determined that RlemTPS2 encodes a sabinene synthase. Sabinene has antifungal activity toward Alternaria alternata. Furthermore, site-directed mutagenesis identified one amino acid, Ile, located at the front of the metal ion binding motif as an important residue for the product specificity of sabinene synthase.

Author
著者 Kohzaki Keisuke
著者(ヨミ) コウザキ ケイスケ
著者(別表記) 神崎 啓亮
著者 Gomi Kenji
著者(ヨミ) ゴミ ケンジ
著者(別表記) 五味 剣二
著者 Yamasaki Yumiko
著者(ヨミ) ヤマサキ ユミコ
著者(別表記) 山崎 祐末子
著者 Ozawa Rika
著者(ヨミ) オザワ リカ
著者(別表記) 小澤 理香
著者 Takabayashi Junji
著者(ヨミ) タカバヤシ ジュンジ
著者(別表記) 高林 純示
著者 Akimitsu Kazuya
著者(ヨミ) アキミツ カズヤ
著者(別表記) 秋光 和也
Publication Title
Journal of plant physiology
Volume
166
Issue
15
Start Page
1700
End Page
1704
Publisher
Elsevier
Published Date
20091015
ISSN
0176-1617
NCID
AA10522948
DOI
10.1016/j.jplph.2009.04.003
Resource Type
Journal Article
Language
eng
Resource URL
http://dx.doi.org/10.1016/j.jplph.2009.04.003
Rights
Copyright c 2009 Elsevier Ltd
Text Version
author
Set
香川大学
Copyright (C) 2009 Kagawa University All rights reserved.