Expression and role of GPR87 in urothelial carcinoma of the bladder

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タイトル
Expression and role of GPR87 in urothelial carcinoma of the bladder
ファイル
内容記述

Abstract

The orphan GPR87 has recently been matched with its ligand LPA, which is a lipid mediator with multiple physiological functions, including cancer cell proliferation. This study aimed to clarify the role of GPR87 in urothelial carcinoma of the bladder. GPR87 expression was assessed in seven human bladder cancer cell lines. A replication-deficient recombinant adenoviral vector expressing shRNA targeting GPR87 (Ad-shGPR87), was constructed. Gene silencing was carried out using Ad-shGPR87. Immunohistochemical analysis was performed for transurethral resection of bladder tumor samples from 71 patients with non-muscle-invasive bladder cancer. We observed GPR87 expression in five of the seven cell lines, and silencing GPR87 gene expression significantly reduced cell viability. GPR87 expression was positive in 38 (54%) of 71 tumors. Ki-67 index was associated with positive GPR87 staining status (p < 0.0001). Patients with GPR87-positive tumors had shorter intravesical recurrence-free survival than those with GPR87-negative tumors (p = 0.010). Multivariate analysis revealed that GPR87 staining status was an independent prognostic parameter for intravesical recurrence (p = 0.041). Progression from non-muscle-invasive to muscle-invasive tumor was more frequently observed in patients with GPR87-positive tumors, although this trend did not reach statistical significance (p = 0.056). These results warrant further prospective studies to clarify the role of GPR87 expression in intravesical recurrence and progression in bladder cancer.

Keywords:
GPR87, non-muscle-invasive bladder cancer, intravesical recurrence, progression

(医博甲575)

著者
著者 岡添 誉
著者(ヨミ) オカゾエ ホマレ
著者(別表記) Okazoe Homare
掲載誌
International Journal of Molecular Sciences
掲載誌(別表記)
Int J Mol Sci.
14
6
開始ページ
12367
終了ページ
12379
出版者
MDPI
出版年月日
2013-06-10
ISSN
1661-6596
NCID
AA12038549
PMID
23752273
DOI
10.3390/ijms140612367
資料タイプ
学位論文
言語
英語
関連情報
PMCID: PMC3709790
関連情報URL
https://doi.org/10.3390/ijms140612367
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709790/
権利関係
Copyright © 2013 by the authors; licensee MDPI, Basel, Switzerland
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
DOI: https://doi.org/10.3390/ijms140612367
博士論文(全文を含む)
学位授与番号
博甲第575号
学位授与年月日
2013-09-26
学位名
博士(医学)
学位授与機関
香川大学
区分
香川大学
Copyright (C) 2009 Kagawa University All rights reserved.