An immunohistochemical study of human platelets using a rabbit antibody against H18-K24 of apolipoprotein CIII (HATKTAK)

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/27478
Title
An immunohistochemical study of human platelets using a rabbit antibody against H18-K24 of apolipoprotein CIII (HATKTAK)
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Description

Abstract

H18-K24 of human apolipopotein CIII (Apo CIII) (HATKTAK) is an activator of the macromolecular activators of phagocytosis from platelets (MAPPs). Using a rabbit antibody against HATKTAK, we performed an immunohistochemical study of human platelets. Indirect ELISA showed that this antibody reacts with Apo CIII-derived peptides with a C-terminal of HATKTAK, but not with Apo CIII. Immunoelectron microscopy revealed that reaction of anti-HATKTAK antibody occurred in the pseudopods of activated platelets. In blood coagula produced from the peripheral blood and formalin-fixed after various incubation periods, reaction of this antibody with platelets appeared rapidly with a peak at 3 to 6 h of incubation, and then diminished gradually. Leukocytes in the blood coagula were stained strongly positive. In tissue sections, fresh thrombi and hemorrhages with slight fibrin formation revealed a positive response of platelets to anti-HATKTAK antibody, whereas older ones with leukocytic infiltration, fibrin formation and organization did not. In addition to platelets, endothelial cells and leukocytes were stained positive by anti-HATKTAK antibody. All of the positive reactions by anti-HATKTAK antibody disappeared or diminished by co-incubation with HATKTAK. In conclusion, the anti-HATKTAK antibody reveals platelets during the early phase of activation.

KEYWORDS:

antibody; apolipoprotein CIII; hemorrhage; peptide coagula; platelets; thrombi

(医博乙266)

Author
著者 山中 隆夫
著者(ヨミ) ヤマナカ タカオ
著者(別表記) Yamanaka Takao
Publication Title
Pathology International
Publication Title Alternative
Pathol Int.
Volume
63
Issue
8
Start Page
398
End Page
407
Publisher Aalternative
Japanese Society of Pathology
日本病理学会
Wiley
Published Date
2013-08
ISSN
1320-5463
NCID
AA10984364
PMID
23957915
DOI
10.1111/pin.12083
Resource Type
Thesis or Dissertation
Language
eng
Resource URL
https://doi.org/10.1111/pin.12083
Text Version
none
Grant ID
博乙第266号
Grant Date
2013-11-20
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
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