An immunohistochemical study of human platelets using a rabbit antibody against H18-K24 of apolipoprotein CIII (HATKTAK)
URI | http://shark.lib.kagawa-u.ac.jp/kuir/metadata/27478 | ||||||
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Title |
An immunohistochemical study of human platelets using a rabbit antibody against H18-K24 of apolipoprotein CIII (HATKTAK)
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Description |
Abstract H18-K24 of human apolipopotein CIII (Apo CIII) (HATKTAK) is an activator of the macromolecular activators of phagocytosis from platelets (MAPPs). Using a rabbit antibody against HATKTAK, we performed an immunohistochemical study of human platelets. Indirect ELISA showed that this antibody reacts with Apo CIII-derived peptides with a C-terminal of HATKTAK, but not with Apo CIII. Immunoelectron microscopy revealed that reaction of anti-HATKTAK antibody occurred in the pseudopods of activated platelets. In blood coagula produced from the peripheral blood and formalin-fixed after various incubation periods, reaction of this antibody with platelets appeared rapidly with a peak at 3 to 6 h of incubation, and then diminished gradually. Leukocytes in the blood coagula were stained strongly positive. In tissue sections, fresh thrombi and hemorrhages with slight fibrin formation revealed a positive response of platelets to anti-HATKTAK antibody, whereas older ones with leukocytic infiltration, fibrin formation and organization did not. In addition to platelets, endothelial cells and leukocytes were stained positive by anti-HATKTAK antibody. All of the positive reactions by anti-HATKTAK antibody disappeared or diminished by co-incubation with HATKTAK. In conclusion, the anti-HATKTAK antibody reveals platelets during the early phase of activation. KEYWORDS: antibody; apolipoprotein CIII; hemorrhage; peptide coagula; platelets; thrombi (医博乙266) |
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Author |
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Publication Title |
Pathology International
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Publication Title Alternative |
Pathol Int.
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Volume |
63
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Issue |
8
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Start Page |
398
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End Page |
407
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Publisher Aalternative |
Japanese Society of Pathology
日本病理学会
Wiley
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Published Date |
2013-08
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ISSN |
1320-5463
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NCID |
AA10984364
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PMID |
23957915
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DOI |
10.1111/pin.12083
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Resource Type |
Thesis or Dissertation
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Language |
eng
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Resource URL |
https://doi.org/10.1111/pin.12083
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Text Version |
none
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Grant ID |
博乙第266号
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Grant Date |
2013-11-20
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Degree Name |
博士(医学)
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Grantor |
香川大学
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Set |
香川大学
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