The rare sugar D-allose has a reducing effect against ischemia-reperfusion injury on the rat abdominal skin island flap model

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/27479
Title
The rare sugar D-allose has a reducing effect against ischemia-reperfusion injury on the rat abdominal skin island flap model
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Description

Abstract
BACKGROUND:

Recently, one of the rare sugars, D-allose, has received attention from many researchers because of its availability for mass production and its various physiological functions. Among these, an antioxidative effect has been strongly suggested. In this study, we investigated whether this effect is also applicable to the field of skin surgery.
METHODS:

In ischemia-reperfusion injury model using the rat abdominal skin island flap (male Wistar rats, n = 110), D-allose was injected intravenously 15 min before 8-h ischemia. The survival area (%) was measured by digital photographic assessment 1 wk after surgery, and multiple comparisons (Fisher's protected least significant difference) were carried out. Histopathological examination (neutrophilic infiltration into dermis in hematoxylin and eosin stain) and immunostaining (of ectodermal dysplasia-1 (ED1)-positive cells/flap) were assessed. Myeloperoxidase (MPO) activity in the skin flap (sampling at the time of 8 h after reperfusion) was measured spectrophotometrically, and Student t-test was performed.
RESULTS:

D-allose extended the survival of the remaining flaps, and a dose greater than 30 mg (0.1 mg/g) was necessary to be effective. The flap survival rates in the 30, 60, and 150 mg groups were significantly higher than that in the control (saline) group: 75.87 ± 5.90, 79.27 ± 7.81, and 77.87 ± 6.20 versus 50.53 ± 9.66, respectively (P < 0.05). ED1-positive cells/flap in 60 mg of D-allose and control (saline) were 78 ± 25.7 versus 124 ± 15.8, respectively (P = 0.08). The MPO activity in the D-allose 60 mg group was 0.40 ± 0.04, and that in the control (saline) was 0.72 ± 0.12. D-allose significantly reduced the skin tissue MPO activity (P < 0.05) compared with that in the control (saline) group.
CONCLUSIONS:

We proved that D-allose has a reducing effect against ischemia-reperfusion injury on the skin island flap model, and the mechanism is related to inhibiting the activity of neutrophils in the skin tissues. Compared with chemo-synthetic materials, rare sugars are safer for our bodies as well as the environment; therefore, this rare sugar project is expected to lead to the development of a safer antioxidant for skin flap surgery.

KEYWORDS:

Antioxidant; D-allose; Ischemia-reperfusion injury; Myeloperoxidase (MPO); Neutrophil; Rare sugar; Skin island flap

(医博乙267)

Author
著者 宗内 巌
著者(ヨミ) ムネウチ ガン
著者(別表記) Muneuchi Gan
Publication Title
Journal of Surgical Research
Publication Title Alternative
J Surg Res.
Volume
183
Issue
2
Start Page
976
End Page
981
Publisher
Association for Academic Surgery
Academic Press
Elsevier
Published Date
2013-03-21
ISSN
0022-4804
NCID
AA00708021
PMID
23541813
DOI
10.1016/j.jss.2013.03.006
Resource Type
Thesis or Dissertation
Language
eng
Resource URL
https://doi.org/10.1016/j.jss.2013.03.006
Rights
Copyright © 2013 Elsevier Inc.
この博士論文の本文については、次のエルゼビアの著作権ポリシーの規定により公開しています。「博士論文の場合は、エンバーゴ期間に関係なく機関リポジトリに出版社版を公開することができます。」 The text of this doctoral dissertation is published according to the following Elsevier copyright policy. "In the case of doctoral dissertations, publishers can be published in institutional repositories regardless of the Embargo period."
"This is the Publisher's version of the following article: The rare sugar D-allose has a reducing effect against ischemia-reperfusion injury on the rat abdominal skin island flap model; Journal of Surgical Research; Volume 183 Issue 2 (2013) Pages 976-981; doi:10.1016/j.jss.2013.03.006, which has been published in final form at https://doi.org/10.1016/j.jss.2013.03.006 . "
Text Version
ETD
Grant ID
博乙第267号
Grant Date
2013-12-18
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
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