Profile of microRNAs associated with aging in rat liver

( Max 2000 Items )
URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/27634
Title
Profile of microRNAs associated with aging in rat liver
File
Description

Abstract

Recent studies suggest that small non‑coding microRNAs (miRNAs or miRs) play an important role in the regulation of genes involved in various cellular and developmental processes. However, the expression of miRNAs during the aging process remains largely unknown. The aim of the present study was to analyze miRNA expression profiles in rat livers during the aging process. The livers of male Wistar rats at different stages of development (fetal, aged 3 days, and 1, 2, 4, 8 and 36 weeks of age) were used. Total RNA was extracted from the livers. We analyzed the expression levels of 679 rat miRNA probes. In addition, immunohistochemical staining for proliferating cell nuclear antigen (PCNA) was performed. Several up- and downregulated miRNAs were identified in the rat livers at 7 different fetal developmental stages and at 36 weeks of age. We observed the upregulation of miR‑29a, miR‑29c, miR‑195 and miR‑497, whereas miR‑301a, miR‑148b-3p, miR‑7a, miR‑93, miR‑106b, miR‑185, miR‑450a, miR‑539 and miR‑301b were downregulated in the aging rat livers. The number of PCNA-positive hepatocytes was decreased with age. In conclusion, our findings suggest that these up- and downregulated miRNAs play an important role in aging by regulating cell cycles that are involved in liver senescence. Further investigation is required to reveal additional target genes of the miRNAs expressed in the liver and the roles of miRNAs in the developmental process of aging in the liver.

(医博甲595)

Author
著者 三村 志麻
著者(ヨミ) ミムラ シマ
著者(別表記) Mimura shima
Publication Title
International Journal of Molecular Medicine
Publication Title Alternative
Int J Mol Med.
Volume
34
Issue
4
Start Page
1065
End Page
1072
Publisher
Spandidos Publications
Published Date
2014-08-11
ISSN
1107-3756
NCID
AA11445762
PMID
25118807
DOI
10.3892/ijmm.2014.1892
Resource Type
Thesis or Dissertation
Language
eng
Resource URL
https://doi.org/10.3892/ijmm.2014.1892
Rights
Copyright © Spandidos Publications 2014.
"This is the Publisher's version of the following article: Profile of microRNAs associated with aging in rat liver;International Journal of Molecular Medicine; Volume 34 Issue 4 (2014) Pages: 1065-1072; doi: 10.3892/ijmm.2014.1892, which has been published in final form at https://doi.org/10.3892/ijmm.2014.1892 . "
Text Version
ETD
Grant ID
博甲第595号
Grant Date
2014-06-25
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
Copyright (C) 2009 Kagawa University All rights reserved.