MicroRNA profiles following metformin treatment in a mouse model of non-alcoholic steatohepatitis

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/27636
Title
MicroRNA profiles following metformin treatment in a mouse model of non-alcoholic steatohepatitis
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Description

Abstract

Non-alcoholic steatohepatitis (NASH) is one of the most common causes of chronic liver disease and is considered to be a causative factor of cryptogenic cirrhosis and hepatocellular carcinoma. microRNAs (miRNAs) are small non-coding RNAs that negatively regulate messenger RNA (mRNA). Recently, it was demonstrated that the aberrant expression of certain miRNAs plays a pivotal role in liver disease. The aim of the present study was to evaluate changes in miRNA profiles associated with metformin treatment in a NASH model. Eight-week-old male mice were fed a methionine- and choline-deficient (MCD) diet alone or with 0.08% metformin for 15 weeks. Metformin significantly downregulated the level of plasma transaminases and attenuated hepatic steatosis and liver fibrosis. The expression of miRNA-376a, miRNA‑127, miRNA-34a, miRNA-300 and miRNA-342-3p was enhanced among the 71 upregulated miRNAs, and the expression of miRNA-122, miRNA-194, miRNA-101b and miRNA-705 was decreased among 60 downregulated miRNAs in the liver of MCD-fed mice when compared with control mice. Of note, miRNA profiles were altered following treatment with metformin in MCD-fed mice. miRNA-376a, miRNA‑127, miRNA-34a, miRNA-300 and miRNA-342-3p were downregulated, but miRNA-122, miRNA-194, miRNA‑101b and miRNA-705 were significantly upregulated in MCD-fed mice treated with metformin. miRNA profiles were altered in MCD-fed mice and metformin attenuated this effect on miRNA expression. Therefore, miRNA profiles are a potential tool that may be utilized to clarify the mechanism behind the metformin-induced improvement of hepatic steatosis and liver fibrosis. Furthermore, identification of targetable miRNAs may be used as a novel therapy in human NASH.

(医博甲597)

Author
著者 桂 明子
著者(ヨミ) カツラ アキコ
著者(別表記) Katsura Akiko
Publication Title
International Journal of Molecular Medicine
Publication Title Alternative
Int J Mol Med.
Volume
35
Issue
4
Start Page
877
End Page
884
Publisher
Spandidos Publications
Published Date
2015-02-06
ISSN
1107-3756
NCID
AA11445762
PMID
25672270
DOI
10.3892/ijmm.2015.2092
Resource Type
Thesis or Dissertation
Language
eng
Relation
PMCID: PMC4356452
Resource URL
https://doi.org/10.3892/ijmm.2015.2092
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4356452/
Rights
Copyright: © Katsura et al.
"This is the Publisher's Version of the following article: MicroRNA profiles following metformin treatment in a mouse model of non-alcoholic steatohepatitis; International Journal of Molecular Medicine; Volume 35 Issue 4 (2015) Pages: 877-884; doi: 10.3892/ijmm.2015.2092, which has been published in final form at https://doi.org/10.3892/ijmm.2015.2092
This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License(CC BY-NC 3.0)(https://creativecommons.org/licenses/by-nc/3.0/). The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
Text Version
ETD
Grant ID
博甲第597号
Grant Date
2014-09-24
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
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