Rac1-dependent lamellipodial motility in prostate cancer PC-3 cells revealed by optogenetic control of Rac1 activity.

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/27637
Title
Rac1-dependent lamellipodial motility in prostate cancer PC-3 cells revealed by optogenetic control of Rac1 activity.
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Description

Abstract

The lamellipodium, an essential structure for cell migration, plays an important role in the invasion and metastasis of cancer cells. Although Rac1 recognized as a key player in the formation of lamellipodia, the molecular mechanisms underlying lamellipodial motility are not fully understood. Optogenetic technology enabled us to spatiotemporally control the activity of photoactivatable Rac1 (PA-Rac1) in living cells. Using this system, we revealed the role of phosphatidylinositol 3-kinase (PI3K) in Rac1-dependent lamellipodial motility in PC-3 prostate cancer cells. Through local blue laser irradiation of PA-Rac1-expressing cells, lamellipodial motility was reversibly induced. First, outward extension of a lamellipodium parallel to the substratum was observed. The extended lamellipodium then showed ruffling activity at the periphery. Notably, PI(3,4,5)P3 and WAVE2 were localized in the extending lamellipodium in a PI3K-dependent manner. We confirmed that the inhibition of PI3K activity greatly suppressed lamellipodial extension, while the ruffling activity was less affected. These results suggest that Rac1-induced lamellipodial motility consists of two distinct activities, PI3K-dependent outward extension and PI3K-independent ruffling.

(医博甲598)

Author
著者 加藤 琢磨
著者(ヨミ) カトウ タクマ
著者(別表記) Kato Takuma
Publication Title
PLoS ONE
Publication Title Alternative
PLoS One.
Volume
9
Issue
5
Start Page
e97749
End Page
e97749
Publisher
Public Library of Science
Published Date
2014-05-21
ISSN
1932-6203
PMID
24848679
DOI
10.1371/journal.pone.0097749
Resource Type
Thesis or Dissertation
Language
eng
Relation
PMCID: PMC4029798
Resource URL
https://doi.org/10.1371/journal.pone.0097749
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4029798/
Rights
Copyright © 2014 Kato et al
This is an open-access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
DOI: https://doi.org/10.1371/journal.pone.0097749
Text Version
ETD
Grant ID
博甲第598号
Grant Date
2014-12-24
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
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