MicroRNA profiles in cisplatin-induced apoptosis of hepatocellular carcinoma cells

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/27870
Title
MicroRNA profiles in cisplatin-induced apoptosis of hepatocellular carcinoma cells
File
Description

Abstract

Cisplatin [cis-diamminedichloroplatinum (II)], is a platinum coordination compound that is commonly used to treat hepatocellular carcinoma (HCC). It is also one of the most compelling anticancer drugs. Recent studies suggest that cisplatin may reduce cancer risk and improve prognosis. However, the antitumor mechanism of cisplatin in several types of cancers, including HCC, has not been elucidated. The goal of the present study was to evaluate the effects of cisplatin on the proliferation of HCC cells in vitro and to determine which microRNAs (miRNAs) are associated with the anticancer effects of cisplatin in vitro. We used various human HCC-derived cell lines to study the effects of cisplatin on human HCC cells. Cisplatin led to a strong dose- and time- dependent inhibition of cell proliferation in HLE, HLF, HuH7, Li-7, Hep3B and HepG2 cells in vitro. Cisplatin also blocked the progression of the cell cycle in the G0/G1 phase, which inhibited cyclin D1 and induced apoptosis. In addition, miRNA expression was markedly altered by treatment with cisplatin in vitro. Therefore, various miRNAs induced by cisplatin may also contribute to the suppression of cellular proliferation and apoptosis. Our results demonstrate that cisplatin inhibits the growth of HCC, possibly through the induction of G1 cell cycle arrest and apoptosis through the alteration of microRNA expression.

(医博甲646)

Author
著者 宮田 美和
著者(ヨミ) ミヤタ ミワ
著者(別表記) Miyata Miwa
Publication Title
International Journal of Oncology
Publication Title Alternative
Int J Oncol.
Volume
47
Issue
2
Start Page
535
End Page
542
Publisher
Spandidos Publications
Published Date
2015-01-05[Epub]
ISSN
1019-6439
NCID
AA10992511
PMID
26060089
DOI
10.3892/ijo.2015.3036
Resource Type
Thesis or Dissertation
Language
eng
Resource URL
https://doi.org/10.3892/ijo.2015.3036
Relation(isVersionOf)
Publisher's Version.
Rights
"This is the Publisher's version of the following article:MicroRNA profiles in cisplatin-induced apoptosis of hepatocellular carcinoma cells.; International Journal of Oncology; Volume 47 Issue 2 (2015) 535-542; doi: 10.3892/ijo.2015.3036 , which has been published in final form at https://doi.org/10.3892/ijo.2015.3036 ."
Text Version
ETD
Grant ID
博甲第646号
Grant Date
2016-09-29
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
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