Suppressive effects of the expectorant drug ambroxol hydrochloride on quartz-induced lung inflammation in F344 rats

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/27876
Title
Suppressive effects of the expectorant drug ambroxol hydrochloride on quartz-induced lung inflammation in F344 rats
File
Description

Abstract

Surfactant proteins (SPs) are essential to respiratory structure and function. The expectorant drug ambroxol hydrochloride is clinically prescribed to stimulate pulmonary surfactant and airway serous secretion. Therefore, ambroxol hydrochloride may affect SP production and pulmonary inflammation. Lung toxicity of fine particles of various materials has been examined previously in our in vivo bioassay using the intratracheal (i.t.) instillation approach. In the present study, we evaluated modulatory effects of ambroxol hydrochloride on quartz-induced lung inflammation in F344 rats. Male 6-week-old F344 rats were exposed by i.t. instillation to 2 mg of quartz particles suspended in 0.2 mL of saline. Ambroxol hydrochloride was administered at 0, 12, and 120 ppm in rat basal diet for 28 days, and then formalin-fixed paraffin-embedded lung, liver, and kidney samples were prepared. No changes in general condition, body and organ weights, or food consumption upon exposure to quartz were noted. The mean ambroxol intake in rats of the 12 ppm group was comparable to the human conventional dose. Histopathology of lung lesions was evaluated, and the degree of inflammation was scored. At 120 ppm, ambroxol hydrochloride significantly decreased individual lung inflammation scores for pulmonary edema and lymph follicle proliferation around the bronchiole, as well as the total inflammation score, in quartz-treated rats. Expression of SP-C in the type II alveolar cells and macrophages was greater in inflammatory lesions than in non-inflamed areas. Ambroxol treatment did not affect expression of SP-B and SP-C. In conclusion, we demonstrated that ambroxol hydrochloride relieves quartz-induced lung inflammation.

KEYWORDS:

ambroxol hydrochloride; instillation; lung inflammation; quartz; rat; surfactant protein

(医博甲654)

Author
著者 蟹江 尚平
著者(ヨミ) カニエ ショウヘイ
著者(別表記) Kanie Shohei
Publication Title
Journal of Toxicologic Pathology
Publication Title Alternative
J Toxicol Pathol.
Volume
30
Issue
2
Start Page
153
End Page
159
Publisher
Japanese Society of Toxicologic Pathology
Japenese Society of Toxicological Pathology
Publisher Aalternative
日本毒性病理学会
Published Date
2016-12-22
ISSN
0914-9198
NCID
AN10232280
PMID
28458453
DOI
10.1293/tox.2016-0050
Resource Type
Thesis or Dissertation
Language
eng
Relation
PMCID: PMC5406594
Resource URL
https://doi.org/10.1293/tox.2016-0050
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5406594/
Relation(isVersionOf)
Publisher's Version.
Rights
Copyright ©2017 The Japanese Society of Toxicologic Pathology
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/).
DOI: https://doi.org/10.1293/tox.2016-0050
Text Version
ETD
Grant ID
博甲第654号
Grant Date
2017-03-24
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
Copyright (C) 2009 Kagawa University All rights reserved.