Suppressive effects of the expectorant drug ambroxol hydrochloride on quartz-induced lung inflammation in F344 rats

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/27876
タイトル
Suppressive effects of the expectorant drug ambroxol hydrochloride on quartz-induced lung inflammation in F344 rats
ファイル
内容記述

Abstract

Surfactant proteins (SPs) are essential to respiratory structure and function. The expectorant drug ambroxol hydrochloride is clinically prescribed to stimulate pulmonary surfactant and airway serous secretion. Therefore, ambroxol hydrochloride may affect SP production and pulmonary inflammation. Lung toxicity of fine particles of various materials has been examined previously in our in vivo bioassay using the intratracheal (i.t.) instillation approach. In the present study, we evaluated modulatory effects of ambroxol hydrochloride on quartz-induced lung inflammation in F344 rats. Male 6-week-old F344 rats were exposed by i.t. instillation to 2 mg of quartz particles suspended in 0.2 mL of saline. Ambroxol hydrochloride was administered at 0, 12, and 120 ppm in rat basal diet for 28 days, and then formalin-fixed paraffin-embedded lung, liver, and kidney samples were prepared. No changes in general condition, body and organ weights, or food consumption upon exposure to quartz were noted. The mean ambroxol intake in rats of the 12 ppm group was comparable to the human conventional dose. Histopathology of lung lesions was evaluated, and the degree of inflammation was scored. At 120 ppm, ambroxol hydrochloride significantly decreased individual lung inflammation scores for pulmonary edema and lymph follicle proliferation around the bronchiole, as well as the total inflammation score, in quartz-treated rats. Expression of SP-C in the type II alveolar cells and macrophages was greater in inflammatory lesions than in non-inflamed areas. Ambroxol treatment did not affect expression of SP-B and SP-C. In conclusion, we demonstrated that ambroxol hydrochloride relieves quartz-induced lung inflammation.

KEYWORDS:

ambroxol hydrochloride; instillation; lung inflammation; quartz; rat; surfactant protein

(医博甲654)

著者
著者 蟹江 尚平
著者(ヨミ) カニエ ショウヘイ
著者(別表記) Kanie Shohei
掲載誌
Journal of Toxicologic Pathology
掲載誌(別表記)
J Toxicol Pathol.
30
2
開始ページ
153
終了ページ
159
出版者
Japanese Society of Toxicologic Pathology
Japenese Society of Toxicological Pathology
出版者(別表記)
日本毒性病理学会
出版年月日
2016-12-22
ISSN
0914-9198
NCID
AN10232280
PMID
28458453
DOI
10.1293/tox.2016-0050
資料タイプ
学位論文
言語
英語
関連情報
PMCID: PMC5406594
関連情報URL
https://doi.org/10.1293/tox.2016-0050
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5406594/
関連情報(isVersionOf)
Publisher's Version.
権利関係
Copyright ©2017 The Japanese Society of Toxicologic Pathology
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/).
DOI: https://doi.org/10.1293/tox.2016-0050
博士論文(全文を含む)
学位授与番号
博甲第654号
学位授与年月日
2017-03-24
学位名
博士(医学)
学位授与機関
香川大学
区分
香川大学
Copyright (C) 2009 Kagawa University All rights reserved.