D-tagatose inhibits the growth and biofilm formation of Streptococcus mutans

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/27877
Title
D-tagatose inhibits the growth and biofilm formation of Streptococcus mutans
File
Description

Abstract

Dental caries is an important global health concern and Streptococcus mutans has been established as a major cariogenic bacterial species. Reports indicate that a rare sugar, D‑tagatose, is not easily catabolized by pathogenic bacteria. In the present study, the inhibitory effects of D‑tagatose on the growth and biofilm formation of S. mutans GS‑5 were examined. Monitoring S. mutans growth over a 24 h period revealed that D‑tagatose prolonged the lag phase without interfering with the final cell yield. This growth retardation was also observed in the presence of 1% sucrose, although it was abolished by the addition of D‑fructose. S. mutans biofilm formation was significantly inhibited by growth in sucrose media supplemented with 1 and 4% D‑tagatose compared with that in a culture containing sucrose alone, while S. mutans formed granular biofilms in the presence of this rare sugar. The inhibitory effect of D‑tagatose on S. mutans biofilm formation was significantly more evident than that of xylitol. Growth in sucrose media supplemented with D‑tagatose significantly decreased the expression of glucosyltransferase, exo‑β‑fructosidase and D‑fructose‑specific phosphotransferase genes but not the expression of fructosyltransferase compared with the culture containing sucrose only. The activity of cell‑associated glucosyltransferase in S. mutans was inhibited by 4% D‑tagatose. These results indicate that D‑tagatose reduces water‑insoluble glucan production from sucrose by inhibiting glucosyltransferase activities, which limits access to the free D‑fructose released during this process and retards the growth of S. mutans. Therefore, foods and oral care products containing D‑tagatose are anticipated to reduce the risk of caries by inhibiting S. mutans biofilm formation.

(医博甲655)

Author
著者 Khaleque Hasibul
著者(ヨミ) カレック ハシブル
著者(別表記) Hasibul Khaleque
Publication Title
Molecular Medicine Reports
Publication Title Alternative
Mol Med Rep.
Volume
17
Issue
1
Start Page
843
End Page
851
Publisher
Spandidos Publications
Published Date
2017-11-08
ISSN
1791-2997
PMID
29115611
DOI
10.3892/mmr.2017.8017
Resource Type
Thesis or Dissertation
Language
eng
Relation
PMCID: PMC5780163
Resource URL
https://doi.org/ 10.3892/mmr.2017.8017
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780163/
Relation(isVersionOf)
Publisher's Version.
Rights
Copyright © Hasibul et al.
"This is the Publisher's version of the following article:D-tagatose inhibits the growth and biofilm formation of Streptococcus mutans.; Molecular Medicine Reports; Volume 17 Issue 1 (2017) 843-851; doi: 10.3892/mmr.2017.8017 , which has been published in final form at https://doi.org/ 10.3892/mmr.2017.8017 ."
This is an open access article distributed under the terms of Creative Commons Attribution License.
Text Version
ETD
Grant ID
博甲第655号
Grant Date
2017-03-24
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
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