Prolactin regulatory element-binding protein is involved in the suppression of the adiponectin gene in vivo

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/27879
Title
Prolactin regulatory element-binding protein is involved in the suppression of the adiponectin gene in vivo
File
Description

Abstract
PURPOSE:

Prolactin regulatory element-binding protein (PREB), a member of the WD-repeat protein family, has been recognized as a transcriptional factor that regulates prolactin promoter activity in the anterior pituitary of rats. PREB is expressed not only in the pituitary but also in various other tissues, including the adipose tissue. Previous studies have shown that PREB acts as a transcriptional regulator and suppresses the expression of the adiponectin gene in cultured 3T3L1 preadipocytes. The aim of this study was to further examine the potential role of PREB in adipose tissue in vivo.
METHODS:

Transgenic mice that overexpressing PREB (PREB transgenic mice) were generated. Insulin resistance was evaluated in PREB transgenic mice using glucose and insulin tolerance tests. Adiponectin expression in the adipose tissue was examined by western blot analysis and quantitative polymerase chain reaction (qPCR). The expression levels of stearoyl-CoA desaturase (Scd) and adiponectin receptor 2(ADIPOR2) were quantified by qPCR.
RESULTS:

Glucose and insulin tolerance tests revealed insulin resistance in PREB transgenic mice. Serum adiponectin and leptin concentrations were decreased. Adiponectin gene expression was decreased in the adipose tissue, which was confirmed by the downregulation of the adiponectin-dependent hepatic Scd gene and upregulation of the ADIPOR2 gene in the liver of PREB transgenic mice. We also found that pioglitazone, an agonist for the peroxisome proliferator-activated receptor-r, improved the insulin resistance in the PREB transgenic mice after a 10-day feeding period.
CONCLUSIONS:

These results demonstrated that PREB might contribute to the regulation of adiponectin gene expression in vivo.

KEYWORDS:

Adiponectin; Adipose tissue; Diabetes; Insulin resistance; PREB

(医博甲658)

Author
著者 張 暁舟
著者(ヨミ) サン ショウホウ
著者(別表記)
Publication Title
Journal of Endocrinological Investigation
Publication Title Alternative
J Endocrinol Invest.
Volume
40
Issue
4
Start Page
437
End Page
445
Publisher
Associazione Italiana della Tiroide
Associazione Medici Endocrinologi
Societa Italiana dell'Obesita
Societa Italiana di Andrologia e Medicina della Sessualita
Societa Italiana di Endocrinologia
Societa Italiana di Endocrinologia e Diabetologia Pediatrica
Springer
Published Date
2016-12-02
ISSN
0391-4097
NCID
AA00257680
PMID
27914036
DOI
10.1007/s40618-016-0589-3
Resource Type
Thesis or Dissertation
Language
eng
Resource URL
https://doi.org/10.1007/s40618-016-0589-3
Relation(isVersionOf)
Publisher's Version.
Text Version
none
Grant ID
博甲第658号
Grant Date
2017-03-24
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
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