Beneficial effect of D-allose for isolated islet culture prior to islet transplantation

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/27887
Title
Beneficial effect of D-allose for isolated islet culture prior to islet transplantation
File
Description

Abstract
BACKGROUND:

Pretransplant restoration of islets damaged during isolation remains to be solved. In this study, we examined the effect of D-allose on islets isolated from rat pancreata prior to islet transplantation.
METHODS:

Rat islets isolated from fresh pancreata were cultured overnight in Roswell Park Memorial Institute 1640 solution in the absence (group 1) or presence (group 2) of D-allose. Then we assessed stimulation index of insulin, and cure rate after islet transplantation to diabetic nude mice. We also measured malondialdehyde level and caspase 3 activity of islets after the overnight culture for assessment of the oxidative stress and the apoptosis.
RESULTS:

D-allose significantly improved insulin secretion of islets. The stimulation index in group 2 was significantly higher than in group 1. Cure rate after transplantation in group 2 was higher than in group 1 especially in the first week. The malondialdehyde level in group 2 was significantly lower than in group 1. But the caspase 3 activities in both groups did not differ.
CONCLUSIONS:

D-allose treatment of isolated islet culture prior to transplantation restored islet function and increased successful transplant rate. The results of this study suggested that D-allose improved function of damaged islets through its anti-oxidative activity.

KEYWORDS:

D-allose; Islet transplantation; Rare sugar

(医博甲666)

Author
著者 柏木 裕貴
著者(ヨミ) カシワギ ヒロタカ
著者(別表記) Kashiwagi Hirotaka
Publication Title
Journal of Hepato-Biliary-Pancreatic Sciences
Publication Title Alternative
J Hepatobiliary Pancreat Sci.
Volume
23
Issue
1
Start Page
37
End Page
42
Publisher
Asian-Pacific Hepato-Pancreato-Biliary Association
Japan Biliary Association
Japanese Society of Hepato-Biliary-Pancreatic Surgery
Springer
Wiley
Publisher Aalternative
日本胆道学会
日本肝胆膵外科学会
Published Date
2015-11-26
ISSN
1868-6974
NCID
AA12507209
PMID
26517046
DOI
10.1002/jhbp.298
Resource Type
Thesis or Dissertation
Language
eng
Resource URL
https://doi.org/10.1002/jhbp.298
Relation(isVersionOf)
Publisher's Version.( Wiley Online Library)
Text Version
ETD
Grant ID
博甲第666号
Grant Date
2017-03-24
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
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