腫瘍壊死因子とインターロイキン-12を誘導するスモモ果汁成分に関する研究
URI | http://shark.lib.kagawa-u.ac.jp/kuir/metadata/279 | ||||||||||||||||||||||||
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Title |
腫瘍壊死因子とインターロイキン-12を誘導するスモモ果汁成分に関する研究
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Title Alternative |
Studies on the plum components than induce tumor necrosis factor-α and interleulin-12
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File |
KJ00000065481.pdf
( 630.0 KB )
Open Date
:2012-03-27
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Description |
In order to investigate the food components having immunopotentiating activity, mice (C3H/He Slc and ddY) were administered with peach juice to induce anti-tumor cytokines including tumor necrosis factor (TNF-α) and interleukin-12 (IL-12) in vivo. Membrane-filtered plum juice that had been adjusted pH and osmotic pressure to the same value as that of physiological saline was injected intravenously (iv) into mice (200 μl/mouse). Three hours later, lipopolysaccharide (LPS, 10 μg/mouse) was administered iv, and another 2 hr later the blood was taken from individual mice. For determination of serum TNF-α, cytotoxic ability of this cytokine against L929 cell line was measured. To quantitate serum IL-12,a sandwich enzyme linked immunosorbent assay (ELISA) was used. Strikingly it was shown that TNF-α activity of the serum taken from plum juice-treated mice increased up to 60 to 80-fold when compared with that of untreated mouse group. As for IL-12,10-fold increase was observed in the plum-injected mice. Both TNF-α- and IL-12-inducing factors containing in the plum juice appear to be heat labile. Furthermore, as the cytokine inducing activity in the plum juice was destroyed by pre-treatment with proteases and periodate oxidation as well, it was suggested that the factors might be a glycoprotein. The chemical structure of the TNF-α- and IL-12-inducing factor(s), however, awaits further investigations. |
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Author |
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Publication Title |
香川大学農学部学術報告
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Volume |
53
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Start Page |
51
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End Page |
57
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Publisher |
香川大学農学部
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Published Date |
20010330
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ISSN |
0368-5128
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NCID |
AN00038339
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Resource Type |
Departmental Bulletin Paper
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Language |
jpn
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Text Version |
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Set |
香川大学
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