腫瘍壊死因子とインターロイキン-12を誘導するスモモ果汁成分に関する研究

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/279
Title
腫瘍壊死因子とインターロイキン-12を誘導するスモモ果汁成分に関する研究
Title Alternative
Studies on the plum components than induce tumor necrosis factor-α and interleulin-12
File
Description

In order to investigate the food components having immunopotentiating activity, mice (C3H/He Slc and ddY) were administered with peach juice to induce anti-tumor cytokines including tumor necrosis factor (TNF-α) and interleukin-12 (IL-12) in vivo. Membrane-filtered plum juice that had been adjusted pH and osmotic pressure to the same value as that of physiological saline was injected intravenously (iv) into mice (200 μl/mouse). Three hours later, lipopolysaccharide (LPS, 10 μg/mouse) was administered iv, and another 2 hr later the blood was taken from individual mice. For determination of serum TNF-α, cytotoxic ability of this cytokine against L929 cell line was measured. To quantitate serum IL-12,a sandwich enzyme linked immunosorbent assay (ELISA) was used. Strikingly it was shown that TNF-α activity of the serum taken from plum juice-treated mice increased up to 60 to 80-fold when compared with that of untreated mouse group. As for IL-12,10-fold increase was observed in the plum-injected mice. Both TNF-α- and IL-12-inducing factors containing in the plum juice appear to be heat labile. Furthermore, as the cytokine inducing activity in the plum juice was destroyed by pre-treatment with proteases and periodate oxidation as well, it was suggested that the factors might be a glycoprotein. The chemical structure of the TNF-α- and IL-12-inducing factor(s), however, awaits further investigations.

Author
著者 寺本 哲子
著者(ヨミ) テラモト サトコ
著者(別表記) Teramoto Satoko
著者 福本 展子
著者(ヨミ) フクモト ノブコ
著者(別表記) Hukumoto Nobuko
著者 林 健
著者(ヨミ) ハヤシ ケン
著者(別表記) Hayashi Ken
著者 坂戸 信夫
著者(ヨミ) サカト ノブオ
著者(別表記) Sakato Nobuo
Publication Title
香川大学農学部学術報告
Volume
53
Start Page
51
End Page
57
Publisher
香川大学農学部
Published Date
20010330
ISSN
0368-5128
NCID
AN00038339
Resource Type
Departmental Bulletin Paper
Language
jpn
Text Version
publisher
Set
香川大学
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