Prefabrication of Vascularized Allogenic Bone Graft in a Rat by Implanting a Flow-Through Vascular Pedicle and Basic Fibroblast Growth Factor Containing Hydroxyapatite/Collagen Composite.

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Title
Prefabrication of Vascularized Allogenic Bone Graft in a Rat by Implanting a Flow-Through Vascular Pedicle and Basic Fibroblast Growth Factor Containing Hydroxyapatite/Collagen Composite.
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Description

Abstract

Background Basic fibroblast growth factor (bFGF) is known to stimulate bone formation and angiogenesis. Hydroxyapatite/collagen composite (HAp/Col) is also known to have very strong bone conductive activity. In this study, prefabrication of vascularized allogenic bone (allo-bone) graft was attempted in recipients by implanting vascular bundles from recipients into the transplanted allo-bone graft. Furthermore, the effect of bFGF-containing HAp/Col on the prefabricated vascularized allo-bone graft was investigated. Methods In this study, 32 Sprague-Dawley rats were used as donors, and bone grafts were collected from their femora. Thirty-two Wistar rats (recipients) were divided into four groups, and the allo-bone grafts were transplanted into the thigh region. In the experimental groups, one or both of the flow-through saphenous vascular bundles and 100-μg bFGF-containing HAp/Col were implanted into the medullary cavity of the allo-bone grafts. In the control group, neither was implanted. These rats were sacrificed at 4 weeks after transplantation, and bone formation, angiogenesis, and bone resorption in the transplanted allo-bone grafts were evaluated histologically and genetically. Results Bone formation and angiogenesis in the transplanted allo-bone graft were effectively stimulated by implanting vascular bundles or bFGF-containing HAp/Col on both histological and genetic evaluations compared with the control group. The most significant stimulation was observed in the group in which both were implanted. Bone resorption was not stimulated in any group. Conclusion By implanting a flow-through vascular bundle and bFGF-containing HAp/Col, an ideal vascularized allo-bone graft that had high bone formative and angiogenetic activities and did not stimulate bone resorptive activity was prefabricated.

Keywords
allo-bone graft - bFGF - hydroxyapatite/collagen

(医博甲670)

Author
著者 山口 幸之助
著者(ヨミ) ヤマグチ コウノスケ
著者(別表記) Yamaguchi Konosuke
Publication Title
Journal of Reconstructive Microsurgery
Publication Title Alternative
J Reconstr Microsurg.
Volume
33
Issue
5
Start Page
367
End Page
376
Publisher
American Society for Reconstructive Microsurgery
Thieme Publishing
Published Date
2017-02-24
ISSN
0743-684X
NCID
AA10640401
PMID
28235219
DOI
10.1055/s-0037-1599077
Resource Type
Thesis or Dissertation
Language
eng
Relation
出版社版DOIリンク(URL): https://doi.org/10.1055/s-0037-1599077
出版社版URL: https://www.thieme-connect.de/products/ejournals/abstract/10.1055/s-0037-1599077
Rights
Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.
https://doi.org/10.1055/s-0037-1599077
Text Version
ETD
Grant ID
博甲第670号
Grant Date
2017-06-22
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
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