Ethanolamine utilization supports Clostridium perfringens growth in infected tissues

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28523
Title
Ethanolamine utilization supports Clostridium perfringens growth in infected tissues
File
Description

Abstract

Clostridium perfringens possesses the ethanolamine (EA) utilization (eut) system encoded within the eut operon, which utilizes the EA as a carbon, nitrogen and energy source. To determine the role of the eut system in C. perfringens growth, an in-frame deletion of the eutABC genes was made in strain HN13 to generate the eutABC-deleted mutant strain HY1701. Comparison of HN13 and HY1701 growth in media supplemented with 1.0% glucose and/or 1.0% EA showed that glucose enhanced the growth of both strains, whereas EA enhanced HN13 growth, but not that of HY1701, indicating that the eut system is necessary for C. perfringens to utilize EA. The two-component regulatory system EutVW is needed to induce eut gene expression in response to EA whereas the global virulence regulator VirRS differentially controlled eut gene expression depending on glucose and EA availability. To assess the role of the eut system in vivo, an equal number of HN13 and HY1701 cells were injected into the right thigh muscles of mice. Mice infected with HY1701 showed fewer symptoms than those injected with HN13. The mortality rate of mice infected with HY1701 tended to be lower than for mice infected with HN13. In addition, in infected tissues from mice injected with a mixture of HN13 and HY1701, HN13 outnumbered HY1701. PCR screening demonstrated that C. perfringens isolated from gas gangrene and sporadic diarrhea cases carried both eut genes and the perfringolysin O gene (pfoA) as well as the phospholipase C gene (plc). However, pfoA was not detected in isolates from food poisoning patients and healthy volunteers. Culture supernatants prepared from HN13 grown in media containing 7.5% sheep red blood cells induced significantly higher eutB expression levels compared to those from plc- and/or pfoA-deletion mutants. Together, these results indicate that the eut system plays a nutritional role for C. perfringens during histolytic infection.

KEYWORDS:

Clostridium perfringens; Ethanolamine; Gas gangrene; Growth; Mouse model

(医博甲696)

Author
著者 八木 弘文
著者(ヨミ) ヤギ ヒロフミ
著者(別表記) Yagi Hirofumi
Publication Title
Microbial Pathogenesis
Publication Title Alternative
Microb Pathog.
Volume
119
Start Page
200
End Page
207
Publisher
Academic Press
Elsevier
Published Date
2018-04-11
ISSN
0882-4010
NCID
AA10609161
PMID
29654901
DOI
10.1016/j.micpath.2018.04.017
Resource Type
Thesis or Dissertation
Language
eng
Relation
出版社版DOIリンク : https://doi.org/10.1016/j.micpath.2018.04.017
Resource URL
https://doi.org/10.1016/j.micpath.2018.04.017
Relation(isVersionOf)
Publisher's Version.
Rights
Elsevier Japanより、機関リポジトリでの公開可能確認済み。 / From Elsevier Japan, publication possibility confirmed in an institutional repository.
DOI Link of Publisher's Version : https://doi.org/10.1016/j.micpath.2018.04.017
Text Version
ETD
Grant ID
博甲第696号
Grant Date
2018-09-27
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
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