Comparative analyses of isoforms of the calcium-independent phosphatidylethanolamine N-acyltransferase PLAAT-1 in humans and mice

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28674
Title
Comparative analyses of isoforms of the calcium-independent phosphatidylethanolamine N-acyltransferase PLAAT-1 in humans and mice
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Description

Abstract

N-Acylphosphatidylethanolamines (NAPEs) are a class of glycerophospholipids, which are known as precursors for different bioactive N-acylethanolamines. We previously reported that phospholipase A/acyltransferase-1 (PLAAT-1), which was originally found in mammals as a tumor suppressor, catalyzes N-acylation of phosphatidylethanolamines to form NAPEs. However, recent online database suggested the presence of an uncharacterized isoform of PLAAT-1 with an extra sequence at the N terminus. In the present study, we examined the occurrence, intracellular localization, and catalytic properties of this longer isoform, as well as the original shorter isoform from humans and mice. Our results showed that human tissues express the longer isoform but not the short isoform at all. In contrast, mice expressed both isoforms with different tissue distribution. Unlike the cytoplasmic localization of the shorter isoform, the long isoform was found in both cytoplasm and nucleus, inferring that the extra sequence harbors a nuclear localization signal. As assayed with purified proteins, neither isoform required calcium for full activity. Moreover, the overexpression of each isoform remarkably increased cellular NAPE levels. These results conclude that the new long isoform of PLAAT-1 is a calcium-independent N-acyltransferase existing in both cytoplasm and nucleus and suggest a possible formation of NAPEs in various membrane structures including nuclear membrane. J. Lipid Res 2016. 57: 2051-2060.

KEYWORDS:

HRAS-like suppressor family; N-acylethanolamine; N-acylphosphatidylethanolamine; acyltransferase; endocannabinoids; phospholipase A/acyltransferase-1; phospholipases/A2; phospholipids; phospholipids/biosynthesis; phospholipids/phosphatidylethanolamine

(医博甲711)

Author
著者 Md Zahir Hussain
著者(ヨミ) モハマド ジョヒル フセイン
著者(別表記) Hussain Zahir
Publication Title
Journal of Lipid Research
Publication Title Alternative
J Lipid Res.
Volume
57
Issue
11
Start Page
2051
End Page
2060
Publisher
American Society for Biochemistry and Molecular Biology
Published Date
2016-09-13
ISSN
0022-2275
NCID
AA00701215
PMID
27623847
DOI
10.1194/jlr.M071290
Resource Type
Thesis or Dissertation
Language
eng
Relation
出版社版DOIリンク(URL): https://10.1194/jlr.M071290
PMCID: PMC5087872
Resource URL
https://10.1194/jlr.M071290
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5087872/
Relation(isVersionOf)
Publisher's Version.
Text Version
ETD
Grant ID
博甲第711号
Grant Date
2019-03-24
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
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