GSK-3 inhibitor inhibits cell proliferation and induces apoptosis in human osteosarcoma cells

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28740
Title
GSK-3 inhibitor inhibits cell proliferation and induces apoptosis in human osteosarcoma cells
File
Description

Abstract

Glycogen synthase kinase-3 (GSK-3) is a serine/threonine kinase that functions in numerous signaling pathways initiated by diverse stimuli. The functions of GSK-3 in cancer differ depending on cell type. In the present study, we examined the effects of a specific GSK-3 inhibitor on the regulation of osteosarcoma cell proliferation and apoptosis. Immunohistochemical analysis and real-time reverse transcription-polymerase chain reaction (RT-PCR) were performed to determine the expression pattern of GSK-3 in human osteosarcoma cells. We used the MTS assay, western blotting, measurement of single-stranded DNA and morphological analyses to study the effects of a GSK-3 inhibitor, SB216763 on osteosarcoma cell proliferation and survival. We detected an increase in mRNA expression of GSK-3 and aberrant nuclear accumulation of GSK-3 in the osteosarcoma cells. Pharmacological inhibition of GSK-3 led to a decrease in proliferation and survival of osteosarcoma cells. Inhibition of GSK-3 resulted in a decreased expression of Bcl-2 and a subsequent increase in osteosarcoma cell apoptosis via the mitochondrial pathway. The present study demonstrated that GSK-3 activity is critical for tumorigenicity and cell survival in osteosarcoma cells. Our findings suggest that GSK-3 is a potential therapeutic target for the treatment of human osteosarcoma.

(医博甲622)

Author
著者 西村 英樹
著者(ヨミ) ニシムラ ヒデキ
著者(別表記) Nishimura Hideki
Publication Title
Oncology Reports
Publication Title Alternative
Oncol Rep.
Volume
35
Issue
4
Start Page
2348
End Page
2354
Publisher
Spandidos Publications
Published Date
2016-01-15
ISSN
1021-335X
NCID
AA11016405
PMID
26781995
DOI
10.3892/or.2016.4565
Resource Type
Thesis or Dissertation
Language
eng
Resource URL
https://doi.org/10.3892/or.2016.4565
Rights
Copyright © Spandidos Publications 2016.
"This is the Publisher's version of the following article: GSK-3 inhibitor inhibits cell proliferation and induces apoptosis in human osteosarcoma cells; Oncology Reports; Volume 35 Issue 4 (2016) Pages:2348-2354; doi:10.3892/or.2016.4565, which has been published in final form at https://doi.org/10.3892/or.2016.4565 . "
Text Version
ETD
Grant ID
博甲第622号
Grant Date
2016-03-24
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
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