GSK-3 inhibitor inhibits cell proliferation and induces apoptosis in human osteosarcoma cells
URI | http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28740 | ||||||
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Title |
GSK-3 inhibitor inhibits cell proliferation and induces apoptosis in human osteosarcoma cells
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Description |
Abstract Glycogen synthase kinase-3 (GSK-3) is a serine/threonine kinase that functions in numerous signaling pathways initiated by diverse stimuli. The functions of GSK-3 in cancer differ depending on cell type. In the present study, we examined the effects of a specific GSK-3 inhibitor on the regulation of osteosarcoma cell proliferation and apoptosis. Immunohistochemical analysis and real-time reverse transcription-polymerase chain reaction (RT-PCR) were performed to determine the expression pattern of GSK-3 in human osteosarcoma cells. We used the MTS assay, western blotting, measurement of single-stranded DNA and morphological analyses to study the effects of a GSK-3 inhibitor, SB216763 on osteosarcoma cell proliferation and survival. We detected an increase in mRNA expression of GSK-3 and aberrant nuclear accumulation of GSK-3 in the osteosarcoma cells. Pharmacological inhibition of GSK-3 led to a decrease in proliferation and survival of osteosarcoma cells. Inhibition of GSK-3 resulted in a decreased expression of Bcl-2 and a subsequent increase in osteosarcoma cell apoptosis via the mitochondrial pathway. The present study demonstrated that GSK-3 activity is critical for tumorigenicity and cell survival in osteosarcoma cells. Our findings suggest that GSK-3 is a potential therapeutic target for the treatment of human osteosarcoma. (医博甲622) |
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Publication Title |
Oncology Reports
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Publication Title Alternative |
Oncol Rep.
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Volume |
35
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Issue |
4
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Start Page |
2348
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End Page |
2354
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Publisher |
Spandidos Publications
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Published Date |
2016-01-15
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ISSN |
1021-335X
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NCID |
AA11016405
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PMID |
26781995
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DOI |
10.3892/or.2016.4565
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Resource Type |
Thesis or Dissertation
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Language |
eng
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Resource URL |
https://doi.org/10.3892/or.2016.4565
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Rights |
Copyright © Spandidos Publications 2016.
"This is the Publisher's version of the following article: GSK-3 inhibitor inhibits cell proliferation and induces apoptosis in human osteosarcoma cells; Oncology Reports; Volume 35 Issue 4 (2016) Pages:2348-2354; doi:10.3892/or.2016.4565, which has been published in final form at https://doi.org/10.3892/or.2016.4565 . "
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Text Version |
ETD
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Grant ID |
博甲第622号
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Grant Date |
2016-03-24
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Degree Name |
博士(医学)
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Grantor |
香川大学
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Set |
香川大学
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