Cleansing effect of acidic L-arginine on human oral biofilm

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28743
Title
Cleansing effect of acidic L-arginine on human oral biofilm
File
Description

Abstract
BACKGROUND:

Dental plaque formed on tooth surfaces is a complex ecosystem composed of diverse oral bacteria and salivary components. Accumulation of dental plaque is a risk factor for dental caries and periodontal diseases. L-arginine has been reported to decrease the risk for dental caries by elevating plaque pH through the activity of arginine deiminase in oral bacteria. Here we evaluated the potential of L-arginine to remove established oral biofilms.
METHODS:

Biofilms were formed using human saliva mixed with Brain Heart Infusion broth supplemented with 1 % sucrose in multi-well plates or on plastic discs. After washing the biofilms with saline, citrate (10 mM, pH3.5), or L-arginine (0.5 M, pH3.5), the retained biofilms were analyzed by crystal violet staining, scanning electron microscopy, and Illumina-based 16S rDNA sequencing.
RESULTS:

Washing with acidic L-arginine detached oral biofilms more efficiently than saline and significantly reduced biofilm mass retained in multi-well plates or on plastic discs. Illumina-based microbiota analysis showed that citrate (pH3.5) preferentially washed out Streptococcus from mature oral biofilm, whereas acidic L-arginine prepared with 10 mM citrate buffer (pH3.5) non-specifically removed microbial components of the oral biofilm.
CONCLUSIONS:

Acidic L-arginine prepared with citrate buffer (pH3.5) effectively destabilized and removed mature oral biofilms. The acidic L-arginine solution described here could be used as an additive that enhances the efficacy of mouth rinses used in oral hygiene.

KEYWORDS:

Biofilm; L-arginine; Mouth rinse; Oral microbiome; Saliva

(医博甲625)

Author
著者 多田 彩乃
著者(ヨミ) タダ アヤノ
著者(別表記) Tada Ayano
Publication Title
BMC Oral Health
Publication Title Alternative
BMC Oral Health.
Volume
16
Start Page
40
Publisher
BMC (part of Springer Nature)
Published Date
2016-03-22
ISSN
1472-6831
NCID
AA11839983
PMID
27001253
DOI
10.1186/s12903-016-0194-z
Resource Type
Thesis or Dissertation
Language
eng
Relation
PMCID: PMC4802732
Resource URL
https://doi.org/10.1186/s12903-016-0194-z
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4802732/
Rights
Copyright © Tada et al. 2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
"This is the Accepted version of the following article: Cleansing effect of acidic L-arginine on human oral biofilm; BMC Oral Health; Volume 16 (2016) 40; doi: 10.1186/s12903-016-0194-z, which has been published in final form at https://doi.org/10.1186/s12903-016-0194-z . "
Text Version
ETD
Grant ID
博甲第625号
Grant Date
2016-03-24
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
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