Chloroquine Enhances Rapamycin-induced Apoptosis in MG63 Cells.

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28762
Title
Chloroquine Enhances Rapamycin-induced Apoptosis in MG63 Cells.
File
Description

Abstract
BACKGROUND/AIM:

We previously showed that the use of autophagy inhibitors in combination with chemotherapy can enhance anticancer effects in sarcoma cell lines. In this study, we investigated the combined effect of the autophagy inhibitor chloroquine and the mTOR inhibitor rapamycin on MG63 osteosarcoma cells.
MATERIALS AND METHODS:

Effects of chloroquine and/or rapamycin on cell proliferation were assessed by WST-1 assays. Effects of chloroquine and/or rapamycin on the mTOR pathway components, autophagy, and apoptosis were investigated by western blot, flow cytometry, and fluorescence microscopy using immunocytochemical staining of LC3 and Annexin V-FITC/propidium iodide.
RESULTS:

Rapamycin suppressed cell growth and inhibited the mTOR pathway. Rapamycin promoted autophagy by blocking the mTOR pathway, and chloroquine enhanced apoptosis by blocking autophagy.
CONCLUSION:

Chloroquine enhances the effects of rapamycin in inducing apoptosis via autophagy inhibition in MG63 cells. Thus, the combined therapy of chloroquine and rapamycin may be a potent treatment for osteosarcoma.

KEYWORDS:

Rapamycin; apoptosis; autophagy; chloroquine; osteosarcoma

(医博甲722)

Author
著者 石橋 洋一
著者(ヨミ) イシバシ ヨウイチ
著者(別表記) Ishibashi Yoichi
Publication Title
Anticancer Research
Publication Title Alternative
Anticancer Res.
Volume
39
Issue
2
Start Page
649
End Page
654
Publisher
International Institute of Anticancer Research
Published Date
2019-02
ISSN
0250-7005
NCID
AA10625860
PMID
30711941
DOI
10.21873/anticanres.13159
Resource Type
Thesis or Dissertation
Language
eng
Resource URL
https://doi.org/10.21873/anticanres.13159
Rights
Copyright© 2019, International Institute of Anticancer Research
Non-commercial
"This is the Publisher's Version of the following article: Chloroquine Enhances Rapamycin-induced Apoptosis in MG63 Cells; Anticancer Research; Volume 39 Number 2 (2019) Pages: 649-654; doi: 10.21873/anticanres.13159, which has been published in final form at https://doi.org/10.21873/anticanres.13159 . "
Text Version
ETD
Grant ID
博甲第722号
Grant Date
2019-06-25
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
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