L-histidine augments the oxidative damage against Gram-negative bacteria by hydrogen peroxide

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28765
Title
L-histidine augments the oxidative damage against Gram-negative bacteria by hydrogen peroxide
File
Description

Abstract

Excessive damage to DNA and lipid membranes by reactive oxygen species reduces the viability of bacteria. In the present study, the proliferation of recA‑deficient Escherichia coli strains was revealed to be inhibited by 1% L‑histidine under aerobic conditions. This inhibition of proliferation was not observed under anaerobic conditions, indicating that L‑histidine enhances oxidative DNA damage to E. coli cells. Reverse transcription‑quantitative polymerase chain reaction analysis demonstrated that the expression of recA in E. coli MG1655 increased ~7‑fold following treatment with 10 mM hydrogen peroxide (H2O2) plus 1% L‑histidine, compared with that following exposure to H2O2 alone. L‑histidine increased the genomic fragmentation of E. coli MG1655 following exposure to H2O2. In addition, L‑histidine increased the generation of intracellular hydroxyl radicals in the presence of H2O2 in E. coli cells. Next, our group investigated the disinfection properties of the H2O2 and L‑histidine combination. The combination of 100 mM H2O2 and 1.0% L‑histidine significantly reduced the number of viable cells of extended‑spectrum‑β‑lactamase‑producing E. coli and multidrug‑resistant Pseudomonas aeruginosa, and this treatment was more effective than 100 mM H2O2 alone, but this effect was not observed in methicillin‑resistant Staphylococcus aureus or vancomycin‑resistant Enterococcus faecium. The combination of L‑histidine and H2O2 may be a useful strategy to selectively increase the microbicidal activity of oxidative agents against Gram‑negative bacteria.

(医博乙288)

Author
著者 谷山 多美子
著者(ヨミ) タニヤマ タミコ
著者(別表記) Taniyama Tamiko
Publication Title
International Journal of Molecular Medicine
Publication Title Alternative
Int J Mol Med.
Volume
41
Issue
5
Start Page
2847
End Page
2854
Publisher
Spandidos Publications
Published Date
2018-02-07
ISSN
1107-3756
NCID
AA11445762
PMID
29436593
DOI
10.3892/ijmm.2018.3473
Resource Type
Thesis or Dissertation
Language
eng
Resource URL
https://doi.org/10.3892/ijmm.2018.3473
Rights
Copyright © Spandidos Publications 2018.
"This is the Publisher's Version of the following article: L-histidine augments the oxidative damage against Gram-negative bacteria by hydrogen peroxide; International Journal of Molecular Medicine; Volume 41 Issue 5 (2018) Pages: 2847-2854; doi: 10.3892/ijmm.2018.3473, which has been published in final form at https://doi.org/10.3892/ijmm.2018.3473 . "
Text Version
ETD
Grant ID
博乙第288号
Grant Date
2019-07-17
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
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