In vitroでのニワウメ(Prunus japonica THUNB.)の不定胚および不定芽形成

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/294
Title
In vitroでのニワウメ(Prunus japonica THUNB.)の不定胚および不定芽形成
Title Alternative
INDUCTION OF ADVENTITIOUS EMBRYO AND BUD IN CHINESE BUSH CHERRY (Prunus japonica THUNB.) IN VITRO
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Description

Conditions inducing adventitious embryo and bud from ovule and embryo of Chinese bush cherry (Prununs japonica THUNB.) were studied. For induction of adventitious embryo, MS basal medium containing Gellan gum 0.2 %, sucrose (3, 6, 9 %) and 2, 4-D was used. Ovules collected before flowering did not survive on any medium. In immature embryo, active callus formation was observed on the medium containing 3 % sucrose+15 μM 2, 4-D, while adventitious embryo was induced only 2, 4-D free medium. Although the adventitious embryos were transferred to the hormone-free MS medium after low temperature treatment, they did not grow into plantlet. For induction of adventitious buds, 1/2 MS basal medium containing Gellan gum O.2 %, sucrose 3 % and BA and CPPU (0, 0.2, 2, 10, 20μM), and NAA (0, 0.2,2 μM) was used. From the ovules collected lOdays after flowering, the callus was formed, but adventitious bud was not induced. ln the cotyledons of mature embryo, adventitious buds were formed most actively on the medium with BA 10μM. Addition of NAA suppressed adventitious bud formation. After elongating the adventitious buds on the 1/2 MS medium with 2iP 2.5μM,the base of the shoots were treated with IBA 10 mM and cultured on hormone-free 1/2 MS medium. lOdays after cutting, the shoots started rooting and grew up to the plantlets.

Author
著者 片岡 郁雄
著者(ヨミ) カタオカ イクオ
著者(別表記) KATAOKA Ikuo
著者 植村 智恵
著者(ヨミ) ウエムラ チエ
著者(別表記) UEMURA Chie
著者 別府 賢治
著者(ヨミ) ベップ ケンジ
著者(別表記) BEPPU Kenji
Publication Title
香川大学農学部学術報告
Volume
55
Start Page
19
End Page
24
Publisher
香川大学農学部
Published Date
20030328
ISSN
0368-5128
NCID
AN00038339
Resource Type
Departmental Bulletin Paper
Language
jpn
Text Version
publisher
Set
香川大学
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