Molecular cloning of a root hairless gene rht1 in rice

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/3367
Title
Molecular cloning of a root hairless gene rht1 in rice
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Description

Root hairs, projections from the epidermal cells of the roots, are contributing to water and nutrient uptake and anchorage to the soil. To better understand genetic control of root hair formation in rice, we analyzed root hairless 1 (rth1) mutant that was induced by NaN_3 treatment. SEM observation showed that in rth1 plants, root hair elongation was abolished after the formation of bulge. High-resolution mapping using 2,088 segregants revealed three predicted genes in a 38-kb candidate interval on chromosome 7. Sequences comparison of the three genes between wild-type Oochikara and rth1 detected a nucleotide substitution only in apyrase (OsAPY). This nucleotide substitution (G→A) lies in the junction between the third intron and the forth exon, and results in the splicing anomaly to the rth1 cDNA sequence. Transgenic plants with introduced OsAPY allele restored normal root hairs and plant growth, showing a complementation of rth1 phenotype. We concluded that the root hairless phenotype of rth1 is caused by a mutation of OsAPY. OsAPY appears to be an important gene for root hair elongation and plant growth in rice.

Author
著者 Yuo Takahisa
著者(ヨミ) ユオ タカヒサ
著者(別表記) 湯尾 崇央
著者 Toyota Masanori
著者(ヨミ) トヨタ マサノリ
著者(別表記) 豊田 正範
著者 Ichii Masahiko
著者(ヨミ) イチイ マサヒコ
著者(別表記) 一井 眞比古
著者 Taketa Shin
著者(ヨミ) タケタ シン
著者(別表記) 武田 真
Publication Title
Breeding science
Volume
59
Issue
1
Start Page
13
End Page
20
Publisher
Japanese Society of Breeding
Published Date
200903
ISSN
1344-7610
NCID
AA11353132
Resource Type
Journal Article
Language
eng
Relation URL(isReplacedBy)
http://ci.nii.ac.jp/naid/110007122769
Rights
Japanese Society of Breeding
本文データは学協会の許諾に基づきCiNiiから複製したものである
Text Version
publisher
Set
香川大学
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