Essential Role of Mg^<2+> in Flocculation of Saccharomyces diastaticus

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/451
Title
Essential Role of Mg^<2+> in Flocculation of Saccharomyces diastaticus
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Description

Flocculation of Saccharomyces diastaticus IFO 1958 was studied. Cells of IFO 1958 did not flocculate even in the stationary phase without Mg^<2+> although they began to flocculate strongly 18h after inoculation in the presence of Mg^<2+>. Cycloheximide completely inhibited induction of floe-forming ability of Mg^<2+>-deficient cells. Co-flocculation between flocculent cells and Mg^<2+>-deficient cells was investigated by treatment by proteolytic enzymes and chemical modification. Treatment of Mg^<2+>-deficient cells by proteolytic enzymes did not affect the co-flocculation with flocculent cells. Photo-oxidation or mercaptoethanol-reduction of Mg^<2+>-deficient cells failed to diminish the co-flocculation with flocculent cells while treatment of Mg^<2+>-deficient cells by periodate caused a considerable loss of the co-flocculation. On the other hand, flocculent cells deflocculated by proteolysis or chemical modification of protein component failed to co-flocculate with Mg^<2+>-deficient cells. These findigs suggest that Mg^<2+>-deficient cells are non-flocculent because of lack of protein component essential for flocculation of cells of IFO 1958.

Author
著者 Nishihara Hiroshi
著者(ヨミ) ニシハラ ヒロシ
著者(別表記) 西原 浩
著者 Onishi Chihiro
著者(ヨミ) オオニシ チヒロ
著者(別表記) 大西 千尋
著者 Baba Satoko
著者(ヨミ) ババ サトコ
著者(別表記) 馬場 智子
Publication Title
香川大学教育学部研究報告第Ⅱ部
Volume
56
Issue
1
Start Page
41
End Page
50
Publisher
香川大学教育学部
Published Date
20060331
ISSN
0389-3057
NCID
AN00038226
Resource Type
Departmental Bulletin Paper
Language
eng
Text Version
publisher
Set
香川大学
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