Flocculation of Saccharomyces diastaticus Grown with Starch

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/918
Title
Flocculation of Saccharomyces diastaticus Grown with Starch
File
Description

Cells of Saccharomyces diastaticus IFO 1958 did not flocculate 24h after inoculation in the medium with starch as carbon source but flocculated 48h after inoculation. Cycloheximide completely inhibited induction of floc-forming ability of cells grown for 24h in the medium including starch. The effect of chemical modification of cell surface protein and carbohydrate components on the floc-forming ability of flocculent cells grown with starch for 48h and72 h was studied. Treatment with proteolytic enzymes destroyed the floc-forming ability. Photo-oxidation in the presence of methylene blue and reduction with mercaptoethanol eliminated the flocculent ability. Oxidation with sodium periodate also deprived the cells of floc-forming ability. High concentrations of protein-denaturants brought about reversible deflocculation of the flocculent cells. These findings suggest that cell surface protein and carbohydrate components play essential roles in flocculation of the cells grown with starch.

Author
著者 西原 浩
著者(ヨミ) ニシハラ ヒロシ
著者(別表記) NISHIHARA Hiroshi
著者 三宅 舞
著者(ヨミ) ミヤケ マイ
著者(別表記) MIYAKE Mai
Publication Title
香川大学教育学部研究報告第Ⅱ部
Volume
56
Issue
2
Start Page
75
End Page
82
Publisher
香川大学教育学部
Published Date
20060930
ISSN
0389-3057
NCID
AN00038226
Resource Type
Departmental Bulletin Paper
Language
eng
Text Version
publisher
Set
香川大学
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