A quantitative study on splice variants of N-acylethanolamine acid amidase in human prostate cancer cells and other cells

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28490
タイトル
A quantitative study on splice variants of N-acylethanolamine acid amidase in human prostate cancer cells and other cells
ファイル
内容記述

N-Acylethanolamine acid amidase (NAAA) is a lysosomal enzyme, hydrolyzing various bioactive N-acylethanolamines with a preference for palmitoylethanolamide. Human NAAA mRNA was previously reported to consist of multiple 3'-end splice variants. However, their tissue distributions and roles have not been examined yet. In the present study, we first identified four major splice variants (tentatively referred to as a1, a2, b2, and c2) in a human prostate cancer cell line LNCaP, which were composed of exons 1-11, exons 1-10 and 12, exons 1-9 and 12, and exons 1-8 and 12, respectively. We next developed quantitative polymerase chain reaction methods to individually quantify these NAAA variants as well as collectively measure all the variants. Among various human prostate cancer cells, the total levels of NAAA mRNAs in androgen-sensitive cells like LNCaP were higher than those in androgen-insensitive cells. In all of these prostate cells and other human cells, variants a1 and b2 showed the highest and lowest expression levels, respectively, among the four variants. Interestingly, ratios of the four variants were different by cell type. Variants a1 and a2 encoded the same full-length NAAA protein, which was catalytically active, while b2 and c2 were translated to C-terminally truncated proteins. As expressed in HEK293 cells these truncated forms were detected as catalytically inactive precursor proteins, but not as mature forms. These results revealed wide distribution of multiple variants of NAAA mRNA in various human cells and suggested that the proteins from some variants are catalytically inactive.

著者
著者 佐倉 雄馬
著者(ヨミ) サクラ ユウマ
著者(別表記) Sakura Yuma
掲載誌
Biochimica et Biophysica Acta
1861
12
開始ページ
1951
終了ページ
1958
出版者
Elsevier
出版年月日
2016-10-05
ISSN
0006-3002
PMID
27693242
DOI
10.1016/j.bbalip.2016.09.018
資料タイプ
学位論文
言語
英語
関連情報
出版社版DOIリンク(URL) : https://doi.org/10.1016/j.bbalip.2016.09.018
権利関係
Elsevier Japanより、機関リポジトリでの公開可能確認済み。エンバーゴ期間満了。 / From Elsevier Japan, publication possibility confirmed in an institutional repository. The embargo period has expired.
博士論文(全文を含む)
学位授与番号
博甲第656号
学位授与年月日
2017-03-24
学位名
博士(医学)
学位授与機関
香川大学
区分
香川大学
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