Ryanodine receptors contribute to the induction of ischemic tolerance

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28492
Title
Ryanodine receptors contribute to the induction of ischemic tolerance
File
Description

Ischemic tolerance (IT) is induced by a variety of insults to the brain (e.g., nonfatal ischemia, heat and hypoxia) and it provides a strong neuroprotective effect. Although the mechanisms are still not fully elucidated, Ca(2+) is regarded as a key mediator of IT. Ryanodine receptors (RyRs) are located in the sarcoplasmic/endoplasmic reticulum membrane and are responsible for the release of Ca(2+) from intracellular stores. In brain, neuronal RyRs are thought to play a role in various neuropathological conditions, including ischemia. The purpose of the present study was to investigate the involvement of RyRs in IT. Pretreatment with a RyR antagonist, dantrolene (25mg/kg, i.p), blocked IT in a gerbil global ischemia model, while a RyR agonist, caffeine (100mg/kg, i.p), stimulated the production of IT. In vitro, using rat hippocampal cells, short-term oxygen/glucose deprivation induced preconditioning and RyR antagonists, dantrolene (50 and 100 μM) and ryanodine (100 and 200 μM) prevented it. RyR protein and mRNA levels were transiently decreased after induction of IT. These results suggest that RyRs are involved in the induction of ischemic tolerance.

Author
著者 丸山 恵美
著者(ヨミ) マルヤマ エミ
著者(別表記)
Publication Title
Brain Research Bulletin
Volume
122
Start Page
45
End Page
53
Publisher
Elsevier
Published Date
2016-02-27
ISSN
0361-9230
PMID
26930163
DOI
10.1016/j.brainresbull.2016.02.018
Resource Type
Thesis or Dissertation
Language
eng
Relation
出版社版DOIリンク(URL) : https://doi.org/10.1016/j.brainresbull.2016.02.018
Rights
エルゼビア・ジャパンに著作権ポリシーを確認した結果、リポジトリでの公開可能を確認した。 / As a result of confirming the copyright policy to Elsevier Japan, we confirmed the possibility of publishing in the repository.
Text Version
ETD
Grant ID
博甲第638号
Grant Date
2016-06-28
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
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