Gene expression changes in the retina after systemic administration of aldosterone

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28521
Title
Gene expression changes in the retina after systemic administration of aldosterone
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Description

Abstract
PURPOSE:

Retinal ganglion cell (RGC) loss associated with thinning of the retinal nerve fiber layer without elevated intraocular pressure (IOP) occurs after the systemic administration of aldosterone. Since it is important to determine the mechanism of cell death independent of the IOP, we examined gene expression changes in the retina after the systemic administration of aldosterone.
METHODS:

Following subcutaneous implantation of an osmotic minipump into the mid-scapular region of rats, we administered an 80 μg/kg/day dose of aldosterone. Differences in the gene expression in the retina between normal rats and aldosterone-treated rats were investigated using microarrays. Real-time PCR was used to confirm the differential expression.
RESULTS:

Analysis of the microarray data sets revealed the upregulation of 24 genes and the downregulation of 24 genes of key apoptosis-specific genes. Real-time PCR revealed 4 genes (Cdkn1a, Tbox5, Pf4, Vdr) were upregulated while 12 genes (Acvr1c, Asns, Bard1, Card9, Crh, Fcgr1a, Inhba, Kcnh8, Lck, Phlda1, Ptprc, Sh3rf1) were downregulated.
CONCLUSIONS:

Significant increases and decreases were noted in several genes after the systemic administration of aldosterone. Further studies will need to be undertaken in order to definitively clarify the role of these genes in the eyes of animals with normal-tension glaucoma.

KEYWORDS:

Aldosterone; Glaucoma; Microarray; Retina; Retinal ganglion cell

Author
著者 小野 葵
著者(ヨミ) オノ アオイ
著者(別表記) Ono Aoi
Publication Title
Japanese Journal of Ophthalmology
Publication Title Alternative
日本眼科学会誌
Volume
62
Issue
4
Start Page
499
End Page
507
Publisher
Japanese Ophthalmological Society
Springer
Publisher Aalternative
日本眼科学会
Published Date
2018-04-30
ISSN
0021-5155
PMID
29713904
DOI
10.1007/s10384-018-0595-4
Resource Type
Thesis or Dissertation
Language
eng
Resource URL
https://doi.org/10.1007/s10384-018-0595-4
Text Version
ETD
Grant ID
博甲第697号
Grant Date
2018-09-27
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
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