Gene expression changes in the retina after systemic administration of aldosterone

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28521
タイトル
Gene expression changes in the retina after systemic administration of aldosterone
ファイル
内容記述

Abstract
PURPOSE:

Retinal ganglion cell (RGC) loss associated with thinning of the retinal nerve fiber layer without elevated intraocular pressure (IOP) occurs after the systemic administration of aldosterone. Since it is important to determine the mechanism of cell death independent of the IOP, we examined gene expression changes in the retina after the systemic administration of aldosterone.
METHODS:

Following subcutaneous implantation of an osmotic minipump into the mid-scapular region of rats, we administered an 80 μg/kg/day dose of aldosterone. Differences in the gene expression in the retina between normal rats and aldosterone-treated rats were investigated using microarrays. Real-time PCR was used to confirm the differential expression.
RESULTS:

Analysis of the microarray data sets revealed the upregulation of 24 genes and the downregulation of 24 genes of key apoptosis-specific genes. Real-time PCR revealed 4 genes (Cdkn1a, Tbox5, Pf4, Vdr) were upregulated while 12 genes (Acvr1c, Asns, Bard1, Card9, Crh, Fcgr1a, Inhba, Kcnh8, Lck, Phlda1, Ptprc, Sh3rf1) were downregulated.
CONCLUSIONS:

Significant increases and decreases were noted in several genes after the systemic administration of aldosterone. Further studies will need to be undertaken in order to definitively clarify the role of these genes in the eyes of animals with normal-tension glaucoma.

KEYWORDS:

Aldosterone; Glaucoma; Microarray; Retina; Retinal ganglion cell

著者
著者 小野 葵
著者(ヨミ) オノ アオイ
著者(別表記) Ono Aoi
掲載誌
Japanese Journal of Ophthalmology
掲載誌(別表記)
日本眼科学会誌
62
4
開始ページ
499
終了ページ
507
出版者
Japanese Ophthalmological Society
Springer
出版者(別表記)
日本眼科学会
出版年月日
2018-04-30
ISSN
0021-5155
PMID
29713904
DOI
10.1007/s10384-018-0595-4
資料タイプ
学位論文
言語
英語
関連情報URL
https://doi.org/10.1007/s10384-018-0595-4
博士論文(全文を含む)
学位授与番号
博甲第697号
学位授与年月日
2018-09-27
学位名
博士(医学)
学位授与機関
香川大学
区分
香川大学
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