Histomorphometry of ectopic mineralization using undecalcified frozen bone sections

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28682
Title
Histomorphometry of ectopic mineralization using undecalcified frozen bone sections
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Description

Abstract

To investigate the correlation between mineral formation and enhanced expressions of some proteins using undecalcified frozen bone sections. Histological studies have revealed that some proteins, such as BMP2, BMPR1A, and Connexin 43, are expressed in and around sites of ectopic ossification. However, the relationship between the expressed proteins considered to be associated with the ossification and mineral formation in vivo is not clear. Ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1)-mutant spinal hyperostotic TWY mice and ICR mice as controls were euthanized after calcein labeling, and undecalcified frozen sections were obtained from the middle thoracic spine. Intervertebral disc areas were examined histologically and by measuring calcein-labeled areas and areas showing immunoreactivity for BMP2, BMPR1A, and Connexin 43. Calcein-labeled areas, indicating mineralization in the ectopic mineralization sites, were significantly larger in the mutant mice than in controls. The expression of Connexin 43 was elevated in the annulus fibrosus. Increases in the calcein-labeled areas was not correlated with increases in the areas showing immunoreactivity for Connexin 43 in the annulus fibrosus. There was no statistical correlation between enhanced immunohistochemical expression and elevated calcein-labeled areas. By applying the morphometrical analysis method using undecalcified frozen sections to ENPP1-mutant mice, quantitative evaluation of the mineralization and proteins expressed in the surrounding area in the same animal became possible.

KEYWORDS:

BMP2; BMPR1A; Connexin 43; ENPP1; histomorphometry; mineralization; ossification of the posterior longitudinal ligament; undecalcified frozen section

(医博甲719)

Author
著者 藤原 龍史
著者(ヨミ) フジハラ リュウジ
著者(別表記) Fujihara Ryuji
Publication Title
Microscopy Research and Technique
Publication Title Alternative
Microsc Res Tech.
Volume
81
Issue
1
Start Page
1318
End Page
1324
Publisher
Wiley-Liss
Wiley
Published Date
2018-10-08
ISSN
1059-910X
NCID
AA1082314X
PMID
30295362
DOI
10.1002/jemt.23140
Resource Type
Thesis or Dissertation
Language
eng
Relation
出版社版DOIリンク(URL): https://doi.org/10.1002/jemt.23140
Resource URL
https://doi.org/10.1002/jemt.23140
Rights
"This is the Accepted version of the following article: Histomorphometry of ectopic mineralization using undecalcified frozen bone sections; Microscopy Research and Technique;Volume 81 Number 1 (2018) 1318-1324; doi: 10.1002/jemt.23140, which has been published in final form at https://doi.org/10.1002/jemt.23140 . "
DOI: https://doi.org/10.1002/jemt.23140
エンバーゴ期間満了後の2019年10月8日以降、本文(Accepted Version)を公開します。(Accepted Version will be released from October 8, 2019 after the expiration of the embargo period.)
Text Version
none
Grant ID
博甲第719号
Grant Date
2019-03-24
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
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