Histomorphometry of ectopic mineralization using undecalcified frozen bone sections

( 最大 2000 件 )
URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28682
タイトル
Histomorphometry of ectopic mineralization using undecalcified frozen bone sections
ファイル
内容記述

Abstract

To investigate the correlation between mineral formation and enhanced expressions of some proteins using undecalcified frozen bone sections. Histological studies have revealed that some proteins, such as BMP2, BMPR1A, and Connexin 43, are expressed in and around sites of ectopic ossification. However, the relationship between the expressed proteins considered to be associated with the ossification and mineral formation in vivo is not clear. Ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1)-mutant spinal hyperostotic TWY mice and ICR mice as controls were euthanized after calcein labeling, and undecalcified frozen sections were obtained from the middle thoracic spine. Intervertebral disc areas were examined histologically and by measuring calcein-labeled areas and areas showing immunoreactivity for BMP2, BMPR1A, and Connexin 43. Calcein-labeled areas, indicating mineralization in the ectopic mineralization sites, were significantly larger in the mutant mice than in controls. The expression of Connexin 43 was elevated in the annulus fibrosus. Increases in the calcein-labeled areas was not correlated with increases in the areas showing immunoreactivity for Connexin 43 in the annulus fibrosus. There was no statistical correlation between enhanced immunohistochemical expression and elevated calcein-labeled areas. By applying the morphometrical analysis method using undecalcified frozen sections to ENPP1-mutant mice, quantitative evaluation of the mineralization and proteins expressed in the surrounding area in the same animal became possible.

KEYWORDS:

BMP2; BMPR1A; Connexin 43; ENPP1; histomorphometry; mineralization; ossification of the posterior longitudinal ligament; undecalcified frozen section

(医博甲719)

著者
著者 藤原 龍史
著者(ヨミ) フジハラ リュウジ
著者(別表記) Fujihara Ryuji
掲載誌
Microscopy Research and Technique
掲載誌(別表記)
Microsc Res Tech.
81
1
開始ページ
1318
終了ページ
1324
出版者
Wiley-Liss
Wiley
出版年月日
2018-10-08
ISSN
1059-910X
NCID
AA1082314X
PMID
30295362
DOI
10.1002/jemt.23140
資料タイプ
学位論文
言語
英語
関連情報
出版社版DOIリンク(URL): https://doi.org/10.1002/jemt.23140
関連情報URL
https://doi.org/10.1002/jemt.23140
権利関係
"This is the Accepted version of the following article: Histomorphometry of ectopic mineralization using undecalcified frozen bone sections; Microscopy Research and Technique;Volume 81 Number 1 (2018) 1318-1324; doi: 10.1002/jemt.23140, which has been published in final form at https://doi.org/10.1002/jemt.23140 . "
DOI: https://doi.org/10.1002/jemt.23140
エンバーゴ期間満了後の2019年10月8日以降、本文(Accepted Version)を公開します。(Accepted Version will be released from October 8, 2019 after the expiration of the embargo period.)
該当なし
学位授与番号
博甲第719号
学位授与年月日
2019-03-24
学位名
博士(医学)
学位授与機関
香川大学
区分
香川大学
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