Correlation of 4'-[methyl-11C]-thiothymidine uptake with human equilibrative nucleoside transporter-1 and thymidine kinase-1 expressions in patients with newly diagnosed gliomas

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28683
Title
Correlation of 4'-[methyl-11C]-thiothymidine uptake with human equilibrative nucleoside transporter-1 and thymidine kinase-1 expressions in patients with newly diagnosed gliomas
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Description

Abstract
OBJECTIVE:

We examined expressions of human equilibrative nucleoside transporter-1 (hENT1) and thymidine kinase-1 (TK1), the key enzyme in 4'-[methyl-11C]-thiothymidine (4DST) phosphorylation, to elucidate the mechanism of 4DST uptake in patients with newly diagnosed gliomas.
METHODS:

A total of 19 patients with newly diagnosed gliomas were examined with 4DST PET. Tumor lesions were identified as areas of focally increased uptake, exceeding that of normal brain background. For semi-quantitative analysis, tumor-to-contralateral normal brain tissue (T/N) ratio was determined by dividing the maximal standardized uptake value (SUV) for tumor by that of the mean SUV for reference tissue. The expressions of hENT1, TK1 and Ki-67 in tumor specimens were examined by immunohistochemistry and compared with 4DST T/N ratio.
RESULTS:

All but two gliomas showed focally increased 4DST uptake. All gliomas showed hENT1 staining, except one grade II glioma, which was also not visualized on 4DST PET. A significant correlation was observed between T/N ratio and hENT1 score (ρ = 0.90, p < 0.001). All gliomas showed TK1 staining, except two gliomas which were also not visualized on 4DST PET. There was a significant correlation between T/N ratio and TK1 score (ρ = 0.92, p < 0.001). There was a significant correlation between T/N ratio and Ki-67 index (ρ = 0.50, p < 0.03).
CONCLUSION:

Results of this preliminary study indicate that expressions of hENT1 and TK1 appear to be important determinants of 4DST uptake in newly diagnosed gliomas.

KEYWORDS:

4DST; Glioma; PET; TK1; hENT1

(医博甲718)

Author
著者 高見 康景
著者(ヨミ) タカミ ヤスカゲ
著者(別表記) Yasukage Yasukage
Publication Title
Annals of Nuclear Medicine
Volume
32
Issue
9
Start Page
634
End Page
641
Publisher
Japanese Society of Nuclear Medicine
日本核医学会
Springer
Publisher Aalternative
日本核医学会
Published Date
2018-07-23
ISSN
0914-7187
NCID
AA10708017
PMID
30039191[uid]
DOI
10.1007/s12149-018-1285-5
Resource Type
Thesis or Dissertation
Language
eng
Relation
出版社版DOIリンク(URL): https://doi.org/10.1007/s12149-018-1285-5
Resource URL
https://doi.org/10.1007/s12149-018-1285-5
Rights
DOI: https://doi.org/10.1007/s12149-018-1285-5
博士論文の国立国会図書館及び香川大学図書館での閲覧、複写等の利用制限:有 (Access to the doctoral dissertation in the National Diet Library and Kagawa University Library, such as copying, etc .: Yes)
Text Version
none
Grant ID
博甲718号
Grant Date
2019-03-24
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
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