Correlation of 4'-[methyl-11C]-thiothymidine uptake with human equilibrative nucleoside transporter-1 and thymidine kinase-1 expressions in patients with newly diagnosed gliomas

( 最大 2000 件 )
URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28683
タイトル
Correlation of 4'-[methyl-11C]-thiothymidine uptake with human equilibrative nucleoside transporter-1 and thymidine kinase-1 expressions in patients with newly diagnosed gliomas
ファイル
内容記述

Abstract
OBJECTIVE:

We examined expressions of human equilibrative nucleoside transporter-1 (hENT1) and thymidine kinase-1 (TK1), the key enzyme in 4'-[methyl-11C]-thiothymidine (4DST) phosphorylation, to elucidate the mechanism of 4DST uptake in patients with newly diagnosed gliomas.
METHODS:

A total of 19 patients with newly diagnosed gliomas were examined with 4DST PET. Tumor lesions were identified as areas of focally increased uptake, exceeding that of normal brain background. For semi-quantitative analysis, tumor-to-contralateral normal brain tissue (T/N) ratio was determined by dividing the maximal standardized uptake value (SUV) for tumor by that of the mean SUV for reference tissue. The expressions of hENT1, TK1 and Ki-67 in tumor specimens were examined by immunohistochemistry and compared with 4DST T/N ratio.
RESULTS:

All but two gliomas showed focally increased 4DST uptake. All gliomas showed hENT1 staining, except one grade II glioma, which was also not visualized on 4DST PET. A significant correlation was observed between T/N ratio and hENT1 score (ρ = 0.90, p < 0.001). All gliomas showed TK1 staining, except two gliomas which were also not visualized on 4DST PET. There was a significant correlation between T/N ratio and TK1 score (ρ = 0.92, p < 0.001). There was a significant correlation between T/N ratio and Ki-67 index (ρ = 0.50, p < 0.03).
CONCLUSION:

Results of this preliminary study indicate that expressions of hENT1 and TK1 appear to be important determinants of 4DST uptake in newly diagnosed gliomas.

KEYWORDS:

4DST; Glioma; PET; TK1; hENT1

(医博甲718)

著者
著者 高見 康景
著者(ヨミ) タカミ ヤスカゲ
著者(別表記) Yasukage Yasukage
掲載誌
Annals of Nuclear Medicine
32
9
開始ページ
634
終了ページ
641
出版者
Japanese Society of Nuclear Medicine
日本核医学会
Springer
出版者(別表記)
日本核医学会
出版年月日
2018-07-23
ISSN
0914-7187
NCID
AA10708017
PMID
30039191[uid]
DOI
10.1007/s12149-018-1285-5
資料タイプ
学位論文
言語
英語
関連情報
出版社版DOIリンク(URL): https://doi.org/10.1007/s12149-018-1285-5
関連情報URL
https://doi.org/10.1007/s12149-018-1285-5
権利関係
DOI: https://doi.org/10.1007/s12149-018-1285-5
博士論文の国立国会図書館及び香川大学図書館での閲覧、複写等の利用制限:有 (Access to the doctoral dissertation in the National Diet Library and Kagawa University Library, such as copying, etc .: Yes)
該当なし
学位授与番号
博甲718号
学位授与年月日
2019-03-24
学位名
博士(医学)
学位授与機関
香川大学
区分
香川大学
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