Galectin-9 suppresses the growth of hepatocellular carcinoma via apoptosis in vitro and in vivo

( Max 2000 Items )
URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28731
Title
Galectin-9 suppresses the growth of hepatocellular carcinoma via apoptosis in vitro and in vivo
File
Description

Abstract

Galectin-9, a soluble β-galactoside-binding animal lectin, evokes apoptosis in various human cancer cell lines. The galectin-9 antitumor effect against hepatocellular carcinoma (HCC) is, however, unknown. We investigated whether galectin-9 suppresses HCC growth in vitro and in vivo. We assessed the antitumor effect of galectin-9 on HCC cells by conducting WST-8 assay in vitro and xenograft model analysis in vivo. Galectin-9-induced apoptosis was evaluated by FACS and ELISA in vitro and by TUNEL stain in vivo. Cell cycle alteration was profiled by FACS. Caspases were profiled by colorimetry. MicroRNAs related to the galectin-9 antitumor effects were determined using microarrays, and their antitumor effect was confirmed in a transfection study in vitro. The expression levels of the target proteins of the miRNAs extracted above were analyzed by western blot analysis. To summarize the results, galectin-9 inhibited the growth of the HCC cell lines HLE and Li-7 in vitro and Li-7 in vivo inducing apoptosis. Cell cycle turnover was not arrested in HLE and Li-7 cells in vitro. miR-1246 was similarly extracted both in vitro and in vivo, which sensitized Li-7 cells to apoptosis when transfected into the cells. DYRK1A, a target protein of miR-1246 was downregulated in Li-7 cells. Caspase-9 was upregulated in Li-7 cells in vitro and in vivo. In conclusion, galectin-9 inhibited the growth of HCC cells by apoptosis, but not cell cycle arrest, in vitro and in vivo. miR-1246 mediated signals of galectin-9, possibly through miR-1246-DYRK1A-caspase-9 axis. Galectin-9 might be a candidate agent for HCC chemotherapy.

(医博甲612)

Author
著者 藤田 浩二
著者(ヨミ) フジタ コウジ
著者(別表記) Fujita Koji
Publication Title
International Journal of Oncology
Publication Title Alternative
Int J Oncol.
Volume
46
Issue
6
Start Page
2419
End Page
2430
Publisher
Spandidos Publications
Published Date
2015-03-30
ISSN
1019-6439
NCID
AA10992511
PMID
25823465
DOI
10.3892/ijo.2015.2941
Resource Type
Thesis or Dissertation
Language
eng
Resource URL
https://doi.org/10.3892/ijo.2015.2941
Rights
Copyright © Spandidos Publications 2015.
"This is the Publisher's version of the following article: Galectin-9 suppresses the growth of hepatocellular carcinoma via apoptosis in vitro and in vivo; International Journal of Oncology; Volume 46 Issue 6 (2015) Pages: 2419-2430; doi:10.3892/ijo.2015.2941 , which has been published in final form at https://doi.org/10.3892/ijo.2015.2941 . "
Text Version
ETD
Grant ID
博甲第612号
Grant Date
2015-05-25
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
Copyright (C) 2009 Kagawa University All rights reserved.