Galectin-9 suppresses the growth of hepatocellular carcinoma via apoptosis in vitro and in vivo

( 最大 2000 件 )
URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28731
タイトル
Galectin-9 suppresses the growth of hepatocellular carcinoma via apoptosis in vitro and in vivo
ファイル
内容記述

Abstract

Galectin-9, a soluble β-galactoside-binding animal lectin, evokes apoptosis in various human cancer cell lines. The galectin-9 antitumor effect against hepatocellular carcinoma (HCC) is, however, unknown. We investigated whether galectin-9 suppresses HCC growth in vitro and in vivo. We assessed the antitumor effect of galectin-9 on HCC cells by conducting WST-8 assay in vitro and xenograft model analysis in vivo. Galectin-9-induced apoptosis was evaluated by FACS and ELISA in vitro and by TUNEL stain in vivo. Cell cycle alteration was profiled by FACS. Caspases were profiled by colorimetry. MicroRNAs related to the galectin-9 antitumor effects were determined using microarrays, and their antitumor effect was confirmed in a transfection study in vitro. The expression levels of the target proteins of the miRNAs extracted above were analyzed by western blot analysis. To summarize the results, galectin-9 inhibited the growth of the HCC cell lines HLE and Li-7 in vitro and Li-7 in vivo inducing apoptosis. Cell cycle turnover was not arrested in HLE and Li-7 cells in vitro. miR-1246 was similarly extracted both in vitro and in vivo, which sensitized Li-7 cells to apoptosis when transfected into the cells. DYRK1A, a target protein of miR-1246 was downregulated in Li-7 cells. Caspase-9 was upregulated in Li-7 cells in vitro and in vivo. In conclusion, galectin-9 inhibited the growth of HCC cells by apoptosis, but not cell cycle arrest, in vitro and in vivo. miR-1246 mediated signals of galectin-9, possibly through miR-1246-DYRK1A-caspase-9 axis. Galectin-9 might be a candidate agent for HCC chemotherapy.

(医博甲612)

著者
著者 藤田 浩二
著者(ヨミ) フジタ コウジ
著者(別表記) Fujita Koji
掲載誌
International Journal of Oncology
掲載誌(別表記)
Int J Oncol.
46
6
開始ページ
2419
終了ページ
2430
出版者
Spandidos Publications
出版年月日
2015-03-30
ISSN
1019-6439
NCID
AA10992511
PMID
25823465
DOI
10.3892/ijo.2015.2941
資料タイプ
学位論文
言語
英語
関連情報URL
https://doi.org/10.3892/ijo.2015.2941
権利関係
Copyright © Spandidos Publications 2015.
"This is the Publisher's version of the following article: Galectin-9 suppresses the growth of hepatocellular carcinoma via apoptosis in vitro and in vivo; International Journal of Oncology; Volume 46 Issue 6 (2015) Pages: 2419-2430; doi:10.3892/ijo.2015.2941 , which has been published in final form at https://doi.org/10.3892/ijo.2015.2941 . "
博士論文(全文を含む)
学位授与番号
博甲第612号
学位授与年月日
2015-05-25
学位名
博士(医学)
学位授与機関
香川大学
区分
香川大学
Copyright (C) 2009 Kagawa University All rights reserved.