Galectin-9 suppresses cholangiocarcinoma cell proliferation by inducing apoptosis but not cell cycle arrest

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28734
Title
Galectin-9 suppresses cholangiocarcinoma cell proliferation by inducing apoptosis but not cell cycle arrest
File
Description

Abstract

Cholangiocarcinoma is the most common biliary malignancy and the second most common hepatic malignancy after hepatocellular carcinoma (HCC). Galectin-9 (Gal-9) is a tandem-repeat-type galectin that has recently been shown to exert antiproliferative effects on cancer cells. Therefore, the present study evaluated the effects of Gal-9 on the proliferation of human cholangiocarcinoma cells in vitro as well as the microRNAs (miRNAs) associated with the antitumor effects of Gal-9. Gal-9 suppressed the proliferation of cholangiocarcinoma cell lines in vitro and the growth of human cholangiocarcinoma cell xenografts in nude mice. Our data further revealed that Gal-9 increased caspase‑cleaved keratin 18 (CCK18) levels, and the expression of cytochrome c increased in Gal-9-treated cholangiocarcinoma cell lines. These data suggested that Gal-9 induced cholangiocarcinoma cell apoptosis via the intrinsic apoptosis pathway mediated by caspase-dependent or -independent pathways. In addition, Gal-9 reduced the phosphorylation of the epidermal growth factor receptor (EGFR), insulin-like growth factor and insulin-like growth factor-1 receptor (IGF-1R), hepatocyte growth factor receptor and fibroblast growth factor receptor 3 (FGFR3). These findings suggest that Gal-9 can be a candidate of therapeutic target in the treatment of cholangiocarcinoma.

(医博甲616)

Author
著者 小林 聖幸
著者(ヨミ) コバヤシ キヨユキ
著者(別表記) Kobayashi Kiyoyuki
Publication Title
Oncology Reports
Publication Title Alternative
Oncol Rep.
Volume
34
Issue
4
Start Page
1761
End Page
1770
Publisher
Spandidos Publications
Published Date
2015-08-10
ISSN
1021-335X
NCID
AA11016405
PMID
26260906
DOI
10.3892/or.2015.4197
Resource Type
Thesis or Dissertation
Language
eng
Resource URL
https://doi.org/10.3892/or.2015.4197
Rights
Copyright © Spandidos Publications 2015.
"This is the Publisher's version of the following article: Galectin-9 suppresses cholangiocarcinoma cell proliferation by inducing apoptosis but not cell cycle arrest; Oncology Reports; Volume 34 Issue 4 (2015) Pages: 1761-1770; doi:10.3892/or.2015.4197, which has been published in final form at https://doi.org/10.3892/or.2015.4197 . "
Text Version
ETD
Grant ID
博甲第616号
Grant Date
2015-09-29
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
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