Galectin-9 suppresses cholangiocarcinoma cell proliferation by inducing apoptosis but not cell cycle arrest

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28734
タイトル
Galectin-9 suppresses cholangiocarcinoma cell proliferation by inducing apoptosis but not cell cycle arrest
ファイル
内容記述

Abstract

Cholangiocarcinoma is the most common biliary malignancy and the second most common hepatic malignancy after hepatocellular carcinoma (HCC). Galectin-9 (Gal-9) is a tandem-repeat-type galectin that has recently been shown to exert antiproliferative effects on cancer cells. Therefore, the present study evaluated the effects of Gal-9 on the proliferation of human cholangiocarcinoma cells in vitro as well as the microRNAs (miRNAs) associated with the antitumor effects of Gal-9. Gal-9 suppressed the proliferation of cholangiocarcinoma cell lines in vitro and the growth of human cholangiocarcinoma cell xenografts in nude mice. Our data further revealed that Gal-9 increased caspase‑cleaved keratin 18 (CCK18) levels, and the expression of cytochrome c increased in Gal-9-treated cholangiocarcinoma cell lines. These data suggested that Gal-9 induced cholangiocarcinoma cell apoptosis via the intrinsic apoptosis pathway mediated by caspase-dependent or -independent pathways. In addition, Gal-9 reduced the phosphorylation of the epidermal growth factor receptor (EGFR), insulin-like growth factor and insulin-like growth factor-1 receptor (IGF-1R), hepatocyte growth factor receptor and fibroblast growth factor receptor 3 (FGFR3). These findings suggest that Gal-9 can be a candidate of therapeutic target in the treatment of cholangiocarcinoma.

(医博甲616)

著者
著者 小林 聖幸
著者(ヨミ) コバヤシ キヨユキ
著者(別表記) Kobayashi Kiyoyuki
掲載誌
Oncology Reports
掲載誌(別表記)
Oncol Rep.
34
4
開始ページ
1761
終了ページ
1770
出版者
Spandidos Publications
出版年月日
2015-08-10
ISSN
1021-335X
NCID
AA11016405
PMID
26260906
DOI
10.3892/or.2015.4197
資料タイプ
学位論文
言語
英語
関連情報URL
https://doi.org/10.3892/or.2015.4197
権利関係
Copyright © Spandidos Publications 2015.
"This is the Publisher's version of the following article: Galectin-9 suppresses cholangiocarcinoma cell proliferation by inducing apoptosis but not cell cycle arrest; Oncology Reports; Volume 34 Issue 4 (2015) Pages: 1761-1770; doi:10.3892/or.2015.4197, which has been published in final form at https://doi.org/10.3892/or.2015.4197 . "
博士論文(全文を含む)
学位授与番号
博甲第616号
学位授与年月日
2015-09-29
学位名
博士(医学)
学位授与機関
香川大学
区分
香川大学
Copyright (C) 2009 Kagawa University All rights reserved.