Evaluation of bilirubin displacement effect by acetaminophen in vitro

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28738
Title
Evaluation of bilirubin displacement effect by acetaminophen in vitro
File
Description

Abstract
BACKGROUND:

The use of acetaminophen as a drug for pain control is expected to increase in the neonatal field. The displacement factor of acetaminophen in the reaction in the glucose oxidase peroxidase method is very high, but is also considered to be inaccurate based on physicochemical properties.
METHOD:

Unbound bilirubin was measured using the erythrocyte-bound bilirubin measurement method and glucose oxidase peroxidase method by the addition of acetaminophen or sulfisoxazole. The displacement factor was measured using glucose oxidase peroxidase method with the addition of tert-butyl-p-hydroxyanisole.
RESULTS:

Acetaminophen did not increase erythrocyte-bound bilirubin, and the addition of tert-butyl-p-hydroxyanisole lowered its displacement factor. On the other hand, sulfisoxazole increased erythrocyte-bound bilirubin, while tert-butyl-p-hydroxyanisole did not change its displacement factor.
CONCLUSION:

Acetaminophen is an accelerator of the reaction in the glucose oxidase peroxidase method and does not displace bilirubin from human serum albumin.

KEYWORDS:

Erythrocyte-bound bilirubin; accelerator; glucose oxidase peroxidase method; sulfisoxazole; unbound bilirubin

(医博甲620)

Author
著者 杉野 政城
著者(ヨミ) スギノ マサシロ
著者(別表記) Sugino Masashiro
Publication Title
Annals of Clinical Biochemistry
Publication Title Alternative
Ann Clin Biochem.
Volume
52
Issue
4
Start Page
476
End Page
480
Publisher
Association for Clinical Biochemistry
SAGE Publications
Published Date
2014-12-18
ISSN
0004-5632
NCID
AA00532333
PMID
25525256
DOI
10.1177/0004563214567477
Resource Type
Thesis or Dissertation
Language
eng
Resource URL
https://doi.org/10.1177/0004563214567477
Rights
© The Author(s) 2015 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.
"This is the Accepted version of the following article: Evaluation of bilirubin displacement effect by acetaminophen in vitro; Annals of Clinical Biochemistry; Volume 52 Number Pt 4 (2014) Pages:476-480; doi: 10.1177/0004563214567477, which has been published in final form at https://doi.org/10.1177/0004563214567477 . "
Publisher's Version with DOI: https://doi.org/10.1177/0004563214567477
Text Version
ETD
Grant ID
博甲第620号
Grant Date
2016-03-24
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
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