Inhibitory effect of isoamericanol A from Jatropha curcas seeds on the growth of MCF-7 human breast cancer cell line by G2/M cell cycle arrest

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28742
Title
Inhibitory effect of isoamericanol A from Jatropha curcas seeds on the growth of MCF-7 human breast cancer cell line by G2/M cell cycle arrest
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Description

Abstract

Although various parts of J. curcas (Jatropha curcas L., Euphorbiaceae) have long been used as traditional folk medicines for their antiviral, analgesic, and/or antidotal efficacies, we are the first to investigate the role of anti-carcinogenicity of isoamericanol A (IAA) from the seed extract. Our results showed that IAA is capable of inhibiting cell proliferation in a dose-dependent manner on the human cancer cell lines of MCF-7, MDA-MB231, HuH-7, and HeLa. Flow cytometry analysis showed IAA significantly induces cell cycle arrest at G2/M on MCF-7 cells. At both protein and mRNA levels examined by western blot and real-time PCR, the results revealed increased expression of BTG2 (B-cell translocation gene 2), p21 (p21(WAF1/CIPI) ), and GADD45A (growth arrest and DNA-damage-inducible, alpha) after IAA treatment, but inversed expression in CDK1 (cyclin-dependent kinase 1) and cyclins B1 and B2. All these effects contribute to G2/M cell cycle arrest. Furthermore, these results coincide with the changes in molecular expressions determined by DNA-microarray analysis. Our findings indicate that IAA has an inhibitory effect on cell proliferation of MCF-7 through cell cycle arrest, giving it great potential as a future therapeutic reagent for cancers.

KEYWORDS:

Cancer; Cancer treatment; Health sciences; Pharmaceutical science; Plant biology

(医博甲624)

Author
著者 片木 絢子
著者(ヨミ) カタギ アヤコ
著者(別表記) Katagi Ayako
Publication Title
Heliyon
Publication Title Alternative
Heliyon.
Volume
2
Issue
1
Start Page
e00055
Publisher
Elsevier
Published Date
2016-01-06
ISSN
2405-8440
PMID
27441238
DOI
10.1016/j.heliyon.2015.e00055
Resource Type
Thesis or Dissertation
Language
eng
Relation
PMCID: PMC4945895
Resource URL
https://doi.org/10.1016/j.heliyon.2015.e00055
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4945895/
Rights
Copyright © 2015 The Authors. Published by Elsevier Ltd.
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
"This is the Accepted version of the following article: Inhibitory effect of isoamericanol A from Jatropha curcas seeds on the growth of MCF-7 human breast cancer cell line by G2/M cell cycle arrest; Heliyon; Volume 2, Issue 1 (2016) e00055; doi: 10.1016/j.heliyon.2015.e00055, which has been published in final form at https://doi.org/10.1016/j.heliyon.2015.e00055 . "
Text Version
ETD
Grant ID
博甲第624号
Grant Date
2016-03-24
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
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