Immunohistochemical analysis of transporters related to clearance of amyloid-β peptides through blood-cerebrospinal fluid barrier in human brain.

( 最大 2000 件 )
URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28758
タイトル
Immunohistochemical analysis of transporters related to clearance of amyloid-β peptides through blood-cerebrospinal fluid barrier in human brain.
ファイル
内容記述

Abstract

A large number of previous reports have focused on the transport of amyloid-β peptides through cerebral endothelial cells via the blood-brain barrier, while fewer reports have mentioned the transport through the choroid plexus epithelium via the blood-cerebrospinal fluid barrier. Concrete roles of these two pathways remain to be clarified. In this study, we immunohistochemically examined the expression of transporters/receptors that are supposed to be related to the clearance of amyloid-β peptides in the choroid plexus epithelium, the ventricular ependymal cells and the brain microvessels, using seven autopsied human brains. In the choroid plexus epithelium, immunoreactivity for low-density lipoprotein receptor (LDLR), LDLR-related protein 1 (LRP1), LRP2, formylpeptide receptor-like 1 (FPRL1), ATP-binding cassette (ABC) transporter-A1 (ABCA1), ABCC1 and ABCG4 was seen in 7 of 7 brains, while that for ABCB1, ABCG2, RAGE and CD36 was seen in 0-2 brains. In the ventricular ependymal cells, immunoreactivity for CD36, LDLR, LRP1, LRP2, FPRL1, ABCA1, ABCC1 and ABCG4 was seen in 6-7 brains, while that for ABCB1, ABCG2 and RAGE was seen in 0-1 brain. Immunoreactivity for insulin-degrading enzyme (IDE) was seen in three and four brains in the choroid plexus epithelium and the ventricular ependymal cells, respectively. In addition, immunoreactivity for LDLR, ABCB1 and ABCG2 was seen in over 40 % of the microvessels (all seven brains), and that for FPRL1, ABCA1, ABCC1 and RAGE was seen in over 5 % of the microvessels (4-6 brains), while that for CD36, IDE, LRP1, LRP2 and ABCG4 was seen in less than 5 % of the microvessels (0-2 brains). These findings may suggest that these multiple transporters/receptors and IDE expressed on the choroid plexus epithelium, ventricular ependymal cells and brain microvessels complementarily or cooperatively contribute to the clearance of amyloid-β peptides from the brain.

KEYWORDS:

Amyloid-β; Choroid plexus; Ependymal cell; Transporter

(医博乙274)

著者
著者 松本 晃一
著者(ヨミ) マツモト コウイチ
著者(別表記) Matsumoto Koichi
掲載誌
Histochemistry and Cell Biology
掲載誌(別表記)
Histochem Cell Biol.
144
6
開始ページ
597
終了ページ
561
出版者
Society for Histochemistry
Springer (part of Springer Nature)
出版年月日
2015-10-08
ISSN
0948-6143
NCID
AA11033302
PMID
26449856
DOI
10.1007/s00418-015-1366-7
資料タイプ
学位論文
言語
英語
関連情報URL
https://doi.org/10.1007/s00418-015-1366-7
権利関係
Publisher's Version with DOI: https://doi.org/10.1007/s00418-015-1366-7
該当なし
学位授与番号
博乙第274号
学位授与年月日
2016-02-17
学位名
博士(医学)
学位授与機関
香川大学
区分
香川大学
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