Chloroquine Enhances Rapamycin-induced Apoptosis in MG63 Cells.

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28762
タイトル
Chloroquine Enhances Rapamycin-induced Apoptosis in MG63 Cells.
ファイル
内容記述

Abstract
BACKGROUND/AIM:

We previously showed that the use of autophagy inhibitors in combination with chemotherapy can enhance anticancer effects in sarcoma cell lines. In this study, we investigated the combined effect of the autophagy inhibitor chloroquine and the mTOR inhibitor rapamycin on MG63 osteosarcoma cells.
MATERIALS AND METHODS:

Effects of chloroquine and/or rapamycin on cell proliferation were assessed by WST-1 assays. Effects of chloroquine and/or rapamycin on the mTOR pathway components, autophagy, and apoptosis were investigated by western blot, flow cytometry, and fluorescence microscopy using immunocytochemical staining of LC3 and Annexin V-FITC/propidium iodide.
RESULTS:

Rapamycin suppressed cell growth and inhibited the mTOR pathway. Rapamycin promoted autophagy by blocking the mTOR pathway, and chloroquine enhanced apoptosis by blocking autophagy.
CONCLUSION:

Chloroquine enhances the effects of rapamycin in inducing apoptosis via autophagy inhibition in MG63 cells. Thus, the combined therapy of chloroquine and rapamycin may be a potent treatment for osteosarcoma.

KEYWORDS:

Rapamycin; apoptosis; autophagy; chloroquine; osteosarcoma

(医博甲722)

著者
著者 石橋 洋一
著者(ヨミ) イシバシ ヨウイチ
著者(別表記) Ishibashi Yoichi
掲載誌
Anticancer Research
掲載誌(別表記)
Anticancer Res.
39
2
開始ページ
649
終了ページ
654
出版者
International Institute of Anticancer Research
出版年月日
2019-02
ISSN
0250-7005
NCID
AA10625860
PMID
30711941
DOI
10.21873/anticanres.13159
資料タイプ
学位論文
言語
英語
関連情報URL
https://doi.org/10.21873/anticanres.13159
権利関係
Copyright© 2019, International Institute of Anticancer Research
Non-commercial
"This is the Publisher's Version of the following article: Chloroquine Enhances Rapamycin-induced Apoptosis in MG63 Cells; Anticancer Research; Volume 39 Number 2 (2019) Pages: 649-654; doi: 10.21873/anticanres.13159, which has been published in final form at https://doi.org/10.21873/anticanres.13159 . "
博士論文(全文を含む)
学位授与番号
博甲第722号
学位授与年月日
2019-06-25
学位名
博士(医学)
学位授与機関
香川大学
区分
香川大学
Copyright (C) 2009 Kagawa University All rights reserved.