High-pressure carbon monoxide and oxygen mixture is effective for lung preservation

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URI http://shark.lib.kagawa-u.ac.jp/kuir/metadata/28832
Title
High-pressure carbon monoxide and oxygen mixture is effective for lung preservation
File
Description

Abstract

(1) Background: Heme oxygenase-1 (HO-1) degrades heme and generates carbon monoxide (CO), producing various anti-inflammatory, anti-oxidative, and anti-apoptotic effects. This study aimed to confirm the effects of CO on the ischemia-reperfusion injury (IRI) of donor lungs using a high-pressure gas (HPG) preservation method. (2) Methods: Donor rat and canine lungs were preserved in a chamber filled with CO (1.5 atm) and oxygen (O2; 2 atm) and were ventilated with either CO and O2 mixture (CO/O2 group) or air (air group) immediately before storage. Rat lungs were subjected to heterotopic cervical transplantation and evaluated after reperfusion, whereas canine lungs were subjected to allogeneic transplantation and evaluated. (3) Results: Alveolar hemorrhage in the CO/O2 group was significantly milder than that in the air group. mRNA expression levels of HO-1 remained unchanged in both the groups; however, inflammatory mediator levels were significantly lower in the CO/O2 group than in the air group. The oxygenation of graft lungs was comparable between the two groups, but lactic acid level tended to be higher in the air group. (4) Conclusions: The HO-1/CO system in the HPG preservation method is effective in suppressing IRI and preserving donor lungs.

KEYWORDS:

carbon monoxide; high-pressure gas; ischemia–reperfusion injury; lung preservation

(医博甲728)

Author
著者 藤原 敦史
著者(ヨミ) フジワラ アツシ
著者(別表記) Fujiwara Atsushi
Publication Title
International Journal of Molecular Sciences
Publication Title Alternative
Int J Mol Sci.
Volume
20
Issue
11
Publisher
MDPI
Published Date
2019-06-03
ISSN
1422-0067
NCID
AA12038549
PMID
31163581
DOI
10.3390/ijms20112719
Resource Type
Thesis or Dissertation
Language
eng
Relation
PMCID: PMC6600409
Resource URL
https://doi.org/10.3390/ijms20112719
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600409/
Rights
Copyright © 2019 by the authors.
"This is the Publisher's Version of the following article: High-pressure carbon monoxide and oxygen mixture is effective for lung preservation; International Journal of Molecular Sciences; Volume 20 Issue 11 (2019) doi: 10.3390/ijms20112719, which has been published in final form at https://doi.org/10.3390/ijms20112719 . "
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) International license 4.0 (http://creativecommons.org/licenses/by/4.0/).
doi: https://doi.org/10.3390/ijms20112719
Text Version
ETD
Grant ID
博甲第728号
Grant Date
2019-12-25
Degree Name
博士(医学)
Grantor
香川大学
Set
香川大学
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